Biochemistry
Biochemistry
6th Edition
ISBN: 9781305577206
Author: Reginald H. Garrett, Charles M. Grisham
Publisher: Cengage Learning
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Chapter 29, Problem 13P
Interpretation Introduction

Interpretation:

The length of the DNA segment in the B-form DNA needs to be determined. The fraction of a DNA turns around the core octamer comprises of 50 bp of DNA needs to be calculated. The 50 bp of DNA should be compared to the typical size of eukaryotic promoter modules and response elements needs to be explained.

Concept introduction:

In a solution, the common persistence length is 46−50 nm or 140−150 base pairs (the diameter of polymer is two nm), though will vary considerably. This makes DNA a moderately stiff molecule.

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Solid Wire Models of Cell Division 1- Get four solid wires, 2 long (1 green and 1 red) and two short (1 green and 1 red). This will represent the chromatin in a cell. The wires of the same length are homologous. Red wires will represent the chromatin from the female parent while green wires represent the chromatin from the male parent. Label the chromatin as follows: long green- 1a short green- 2c long red- 1b short red- 2d 2- Let the cell pass S phase. Replicate or duplicate each chromatin fiber. Do this by getting another set of wire identical to the original set. Label as before. 3- Combine the replicated chromatin fiber using masking tape. The tape will also mark the position of the centromere. For the short chromatin, place the tape exactly at the middle while for the long chromatin, place it at a point % from the end of the wire. 4- Assume that the chromosome fibers are folded. This will now represent the chromosomes. OR V
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