Biochemistry
6th Edition
ISBN: 9781305577206
Author: Reginald H. Garrett, Charles M. Grisham
Publisher: Cengage Learning
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Chapter 29, Problem 19P
Figure 29.15 highlights in red the DNA phosphate oxygen atoms. Some of them interact with catabolite activator protein (CAP). What kind of interactions do you suppose predominate, and what kinds of CAP amino acid side chains might be involved in these interactions?
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Given the following diagram of how protein AWESOME1 binds to it's target DNA, describe the
potential effects of each of the 5 mutations shown below. The wild-type sequence of a helix #1
is also shown in the blue box, and all the mutations are in helix #1 (see numbers for identifying
particular residues).
a helix #1
R(1)-V-I-L-Y-F-W-I-M-Y-F-S-H-Y-W-R(16)
#1
Predict the consequence of the following
mutations:
1) Arg(1) to Glu
2) Arg(1) to Ala
3) Phe(6) to lle
4) Trp(7) to Phe
5) Met(9) to Pro
in
Describe the d=features of the following DNA-binding domains and how they interact with DNA.
Helix-turn-Helix
Zinc Finger
Leucine Zipper
Helix-loop-Helix
Christian Anfinsen showed that the enzyme Ribonuclease (RNase) is completely inactivated at high concentrations of beta mercaptoethanol (BME). When BME is removed, it restores only approximately 1% of RNase activity. When a very low concentration of BME is added back to RNase, its activity is restored to nearly 100%. Why?
a
Low concentrations of BME causes disulfide bonds to break, but they randomly reform.
b
High concentrations of BME disrupt all disulfide bonds, which inactivates the enzyme. At low concentrations all of the disulfide bonds reform and BME acts as a cofactor for the enzyme.
c
The enzyme is only active when an intermediate number of disulfide bonds exists. which is achieved only at low concentrations of BME.
d
The low concentration of BME allows the majority of the proteins to adopt their most stable form, which is the active form.
Chapter 29 Solutions
Biochemistry
Ch. 29 - Prob. 1PCh. 29 - The Events in Transcription Initiation Describe...Ch. 29 - Substrate Binding by RNA Polymerase RNA polymerase...Ch. 29 - Comparison of Prokaryotic and Eukaryotic...Ch. 29 - Prob. 5PCh. 29 - Prob. 6PCh. 29 - Prob. 7PCh. 29 - Alternative Splicing Possibilities Suppose exon 17...Ch. 29 - Prob. 9PCh. 29 - Prob. 10P
Ch. 29 - Post-transcriptional Modification of Eukaryotic...Ch. 29 - Prob. 12PCh. 29 - Prob. 13PCh. 29 - The Lariat Intermediate in RNA Splicing Draw the...Ch. 29 - Prob. 15PCh. 29 - Prob. 16PCh. 29 - Prob. 17PCh. 29 - Prob. 18PCh. 29 - Figure 29.15 highlights in red the DNA phosphate...Ch. 29 - Chromatin decompaction is a preliminary step in...
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