Biochemistry
Biochemistry
6th Edition
ISBN: 9781305577206
Author: Reginald H. Garrett, Charles M. Grisham
Publisher: Cengage Learning
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Chapter 29, Problem 6P
Interpretation Introduction

Interpretation:

The length of the promoter in nm, the number of turns of B-DNA in it and the number of nucleosomes connected to it need to be determined.

Concept introduction:

In genetic science, a promoter could be a region of polymer that initiates transcription of a specific sequence. Promoters are set close to the transcription sites of genes, on a similar strand and upstream on the polymer. Promoters are 100−1000 base pairs long.

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Matching Match each item with the correct statement below. Not all terms will be used a. 5' GTP cap f. RFLPs b. Target copy g. sticky ends c. Ligase h. cloned DNA d. Cas-9 i recombinant DNA j. ampicillin e. Host cell 1. A cell (usually a bacteria) that has a gene put in it so the gene can be cloned 2. An example of a post-transcriptional modification of mRNA 3. Sections of DNA that vary between individuals. These can be used for DNA fingerprinting 4. May be used following transformation to kill off cells that a gene didn't enter 5. Molecule of DNA created during PCR that is the desired length 6. A molecule of DNA that comes from two different sources that are spliced together 7. Ends of DNA cut by a restriction enzyme where not all nucleotides are paired (i.e. ends are uneven) 8. Protein that works with CRISPR to cut DNA at a specific spot
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