Biology 2e
2nd Edition
ISBN: 9781947172517
Author: Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher: OpenStax
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Textbook Question
Chapter 14, Problem 5RQ
The experiments by Hershey and Chase helped confirm that DNA was the hereditary material on the basis of the finding that:
- radioactive phage were found in the pellet
- radioactive cells were found in the supernatant
- radioactive sulfur was found inside the cell
- radioactive phosphorus was found in the cell
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The following statements are true about common gene cloning procedures except:
-DNA plasmids can help move around genes into other cells
- Restriction enzymes are very important for cutting up and linking chunks of DNA
-We make it so that genes from plants or animals are expressed in bacteria so the products can be harvested
- DNA plasmids are chunks of chromosomal DNA used for cloning
How could personal DNA testing be beneficial to medicine?
fellow lab worker brings you DNA containing what might be a similar gene in Leopard Geckos (XG). She asks you to see if you can amplify it using the same primers you used in frogs and the Bearded Dragon. You run the PCR and then analyze the product by running the DNA on an agarose gel. As a control, you run out the PCR product you amplified in Bearded Dragons and a DNA “ladder” of DNA pieces of known sizes. The gel results are shown below. Marker = DNA ladder; Lane A = Bearded Dragon PCR product; Lane B = Leopard Gecko PCR results. In the box below, determine the sizes of the PCR products shown in Lanes A & B.
Size of Bearded Dragon PCR product: ______________________________
Sizes of Leopard Gecko PCR products: ______________________
yes or No - based on the results shown in the gel above – would you say the frog PCR primers for gene X amplified the same gene in the Bearded Dragon?
Chapter 14 Solutions
Biology 2e
Ch. 14 - Figure 14.10 In eukaryotic cells, DNA and RNA...Ch. 14 - Figure 14.14 You isolate a cell strain in which...Ch. 14 - Figure 14.21 A fr am eshift mutation that results...Ch. 14 - If DNA of a particular species was analyzed and it...Ch. 14 - The experiments by Hershey and Chase helped...Ch. 14 - Bacterial transformation is a major concern in...Ch. 14 - DNA double helix does not have which of the...Ch. 14 - In eukaryotes, what is the DNA wrapped around?...Ch. 14 - Meselson and Stahl's experiments proved that DNA...Ch. 14 - If the sequence of the 5'-3' strand is AATGCTAC,...
Ch. 14 - How did Meselson and Stahl support Watson and...Ch. 14 - Which of the following components is not involved...Ch. 14 - Which of the following does the enzyme primase...Ch. 14 - In which direction does DNA replication take...Ch. 14 - A scientist randomly mutates the DNA of a...Ch. 14 - The ends of the linear chromosomes are maintained...Ch. 14 - Which of the following is not a true statement...Ch. 14 - During proofreading, which of the following...Ch. 14 - The initial mechanism for repairing nucleotide...Ch. 14 - A scientist creates fruit fly larvae with a...Ch. 14 - Explain Griffith's transformation experiments What...Ch. 14 - Why were radioactive sulfur and phosphorous used...Ch. 14 - When Chargaffwas performing his experiments, the...Ch. 14 - Provide a brief summary of the Sanger sequencing...Ch. 14 - Describe the structure and complementary base...Ch. 14 - Prokaryotes have a single circular chromosome...Ch. 14 - How did the scientific community learn that DNA...Ch. 14 - Imagine the Meselson and Stahl experiments had...Ch. 14 - DNA replication is bidirectional and...Ch. 14 - What are Okazaki fragments and how they are...Ch. 14 - If the rate of replication in a particular...Ch. 14 - Explain the events taking place at the replication...Ch. 14 - What is the role of a primer in DNA replication?...Ch. 14 - Quinolone antibiotics treat bacterial infections...Ch. 14 - How do the linear chromosomes in eukaryotes ensure...Ch. 14 - What is the consequence of mutation of a mismatch...Ch. 14 - An adult with a history of tanning has his genome...
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- Cloning Genes Is a Multistep Process Which enzyme is responsible for covalently linking DNA strands together? a. DNA polymerase b. DNA ligase c. EcoRl d. restriction enzymes e. RNA polymerasearrow_forwardCloning Genes Is a Multistep Process In cloning human DNA, why is it necessary to insert the DNA into a vector such as a bacterial plasmid?arrow_forwardFigure 17.7 You are working in a molecular biology lab and, unbeknownst to you, your lab partner left the foreign genomic DNA that you are planning to clone on the lab bench overnight instead of storing it in the freezer. As a result, it was degraded by nucleases, but still used in the experiment. The plasmid, on the other hand, is fine. What results would you expect from your molecular cloning experiment? There will be no colonies on the bacterial plate. There will be blue colonies only. There will be blue and white colonies. The will be white colonies only.arrow_forward
- Which ingredient will allow the DNA to form cloudy clumps which can be collected with tweezers salt rubbing alcohols detergent solution water What is the purpose of PCR? To copy and then make many copies of a specific region of DNA To copy the entire human genome To make just a few copies of DNA To reveal the sequence of a piece of DNA If enzyme catalase has an optimum pH of 7.0, What do you think will happen en the pH is lowered to 3.0? The rate of its enzyme action is increased. Its enzyme action is not affected at all. The rate of its enzyme action is decreased. Its enzyme action ceases or stops.arrow_forward5) Below is an image that shows both reproductive and therapeutic cloning. Use this image to answer compare and contrast therapeutic and reproductive cloning. Are they used for similar means…etc. Once you have done that answer the question below. a) There are two types of therapeutic cloning. What are they and how are they different?arrow_forwardChoose the statements below which are correct. There may be more than one correct statement. A) primers are required in both PCR and DNA replication B) PCR is done in a tube while DNA replication occurs inside our cells C) PCR and DNA replication both use a type of DNA ploymerase D) PCR will always copy the entire chromosomearrow_forward
- The tuble below shows part of the process of DNA replication Origin al ONA Strand Complementary DNA Strand A-C-C-G-T-A-C T-G-G-A A-T-G What is the most likely effect this could have on genetic information going forward? O There willl be no change in the genetic information that gets transmitted because the complementary strand will never be used as a template O There will not be a change in the genetic information that gets transmitted because the cells having the new section of DNA will cored any mutations O There will be a change in the genetic information that gets transmitted due to the complementary strand being used as a template in fulure DNA replication. OThere will be a change in the genetic information that gets transmitted because the sections of DNA with mistakes will be passed on to make future generalions songerarrow_forwardWhat was the purpose of adding RNase or protease to a DNA extract?arrow_forwardWhich of the following best explains the natural function to the tumor-inducing (Ti) plasmid? It expresses a toxin that causes tumors in insects, making them avoid soil containing Agrobacterium They are used cause tumors in mice, in order to study cancer genetics It is used by Agrobacterium to induce tumors in its plant host, so the plant will make food for the bacteria It is used by geneticists to make transgenic plantsarrow_forward
- A scientist made mistakes during DNA extraction. On his first attempt, he blended the sample too much for a long time. On his 2nd attempt, he used potassium acetate instead of sodium chloride. On his third attempt, he forgot to put C4H8O2.1. Which attempt would be the highest based on increasing DNA purity? what about by increasing DNA yield?2. Explain the effect of his mistakes in each of his attempts on the yield and purity of the DNA sample extracted.3. Which attempt would be the most successful? Why?arrow_forwardDiscuss the experiments Griffith, Avery and Hershey/Chase did in the early 1920-50s that helped determine DNA was the genetic stuff. What was the “take home” from each?arrow_forwardRecombinant DNA molecules are produced by incubating plasmids that have been broken open by a restriction enzyme together with DNA molecules containing complementary sticky ends in the presence of the enzyme ? 1) exonuclease 2) DNA connectase O 3) DNA ligase 4) DNA polymerase O 5) endonucleasearrow_forward
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