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Biology 2e
2nd Edition
ISBN: 9781947172517
Author: Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher: OpenStax
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Textbook Question
Chapter 14, Problem 9RQ
Meselson and Stahl's experiments proved that
- conservative
- semi-conservative
- dispersive
- none of the above
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Students have asked these similar questions
After ONE round of DNA replication in Meselson and Stahl's classic experiment,
the semiconservative model was conclusively proven to be the correct one
all three models of replication were still possibilities
the dispersive model was conclusively proven to be incorrect
the conservative model was conclusively proven to be incorrect
both the conservative and the semiconservative models were still possibilities
O O O
After 3 rounds of replication, what percent of the DNA molecules will contain only 15N?
You take a sample of bacteria with DNA that contains only 14N and allow them to replicate in a medium that contains only 15N. After 3 rounds of replication, what percent of the DNA molecules will contain only 15N? Enter your answer as a whole number without the percent sign. For example, 5% would be entered as 5 and 37% would be entered as 37.
Which of the following is/are true of DNA general recombination?
The process involves invasion of a single-stranded DNA with a free 3'-end into a DNA duplex with the same
sequence (on one strand)
The process involves formation and resolution of a Holliday structure.
The process involves use of a restriction endonuclease.
The process involves synthesis of DNA by DNA polymerase.
Chapter 14 Solutions
Biology 2e
Ch. 14 - Figure 14.10 In eukaryotic cells, DNA and RNA...Ch. 14 - Figure 14.14 You isolate a cell strain in which...Ch. 14 - Figure 14.21 A fr am eshift mutation that results...Ch. 14 - If DNA of a particular species was analyzed and it...Ch. 14 - The experiments by Hershey and Chase helped...Ch. 14 - Bacterial transformation is a major concern in...Ch. 14 - DNA double helix does not have which of the...Ch. 14 - In eukaryotes, what is the DNA wrapped around?...Ch. 14 - Meselson and Stahl's experiments proved that DNA...Ch. 14 - If the sequence of the 5'-3' strand is AATGCTAC,...
Ch. 14 - How did Meselson and Stahl support Watson and...Ch. 14 - Which of the following components is not involved...Ch. 14 - Which of the following does the enzyme primase...Ch. 14 - In which direction does DNA replication take...Ch. 14 - A scientist randomly mutates the DNA of a...Ch. 14 - The ends of the linear chromosomes are maintained...Ch. 14 - Which of the following is not a true statement...Ch. 14 - During proofreading, which of the following...Ch. 14 - The initial mechanism for repairing nucleotide...Ch. 14 - A scientist creates fruit fly larvae with a...Ch. 14 - Explain Griffith's transformation experiments What...Ch. 14 - Why were radioactive sulfur and phosphorous used...Ch. 14 - When Chargaffwas performing his experiments, the...Ch. 14 - Provide a brief summary of the Sanger sequencing...Ch. 14 - Describe the structure and complementary base...Ch. 14 - Prokaryotes have a single circular chromosome...Ch. 14 - How did the scientific community learn that DNA...Ch. 14 - Imagine the Meselson and Stahl experiments had...Ch. 14 - DNA replication is bidirectional and...Ch. 14 - What are Okazaki fragments and how they are...Ch. 14 - If the rate of replication in a particular...Ch. 14 - Explain the events taking place at the replication...Ch. 14 - What is the role of a primer in DNA replication?...Ch. 14 - Quinolone antibiotics treat bacterial infections...Ch. 14 - How do the linear chromosomes in eukaryotes ensure...Ch. 14 - What is the consequence of mutation of a mismatch...Ch. 14 - An adult with a history of tanning has his genome...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Meselson-Stahl first cultured bacterial E. coli cells in a medium containing N15 (heavy nitrogen) for a number of generations. Then, they transferred the cells to a medium containing N14 (light nitrogen). After allowing the cells to grow and complete two rounds of replication, which one of the following choices best describes the properties of double-stranded DNA molecules in the population of cells? One type of double-stranded DNA molecule, all DNA molecules have incorporated both N14 and 15 OTwo types of double-stranded DNA molecules, one double-stranded DNA molecule that has incorporated only N14, and the second double-stranded DNA molecule that has incorporated both N14 and 15 One type of double-stranded DNA molecule, all DNA molecules have incorporated both N14 OTwo types of double-stranded DNA molecules, one double-stranded DNA molecule that has incorporated only N15, and the second double-stranded DNA molecule that has incorporated only N¹4. One type of double-stranded DNA…arrow_forwardWhich of the following DNA repair systems could be used to repair the damage caused by UV light exposure? DNA polymerase proofreading Methyl-directed mismatch repair Nucleotide excision repair Nonhomologous end-joining Homologous recombinationarrow_forwardWhich of the following steps is NOT part of a typical polymerase chain reaction? Choose an answer below: primer annealing ligation of DNA fragments by DNA ligase primer extension by DNA polymerase heat denaturation of double stranded DNA none of the abovearrow_forward
- In 1958, Meselson and Stahl conducted an experiment to determine which of the three proposed methods of DNA replication was correct. Identify the three proposed models for DNA replication. Conservative xxx xxx XXxxx xx xa xxxx xxx xxx Semiconservative Answer Bank xxx XXXX < Dispersive xxxxxxx XxXxXxXxxarrow_forwardThe image below shows the replication bubble of a piece of DNA in the process of replication. However, the image only shows the DNA strands being replicated. Fill in the rest of the elements of the figure, specifically: primers, Okazaki fragments, newly replicated leading strand DNA, as well as the enzymes helicase, primase, DNA polymerase III, DNA polymerase I and ligase. Also be sure to indicate the 5’ and 3’ ends of all nucleic acid polymers.arrow_forwardDNA replicates by which of the following models? a. conservative b. semiconservative c. dispersive d. none of the abovearrow_forward
- Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel, and the different bands are labeled with fluorescent nucleotides and scanned with a laser. b. A laser is used to fluorescently label the nucleotides present within the DNA, the DNA is run on a gel, and then the DNA is broken into fragments. c. Nucleotides are scanned with a laser and incorporated into the DNA that has been separated on a gel, and then the DNA is amplified with PCR. d. Fragments of DNA are produced in a reaction that labels them with any of four different fluorescent dyes, and the fragments then are run on a gel and scanned with a laser. e. DNA is broken down into its constituent nucleotides, and the nucleotides are then run on a gel and purified with a laser.arrow_forwardFigure 17.7 You are working in a molecular biology lab and, unbeknownst to you, your lab partner left the foreign genomic DNA that you are planning to clone on the lab bench overnight instead of storing it in the freezer. As a result, it was degraded by nucleases, but still used in the experiment. The plasmid, on the other hand, is fine. What results would you expect from your molecular cloning experiment? There will be no colonies on the bacterial plate. There will be blue colonies only. There will be blue and white colonies. The will be white colonies only.arrow_forwardWhich of the following things must happen first if DNA is going to be replicated * DNA gets broken into smaller pieces to make the process more manageable DNA gets unzipped, so the two complimentary strands are separated DNA Polymerase adds on bases that match with the original strand DNA gets wound back into chromosomes so it's ready for mitosis Lenovo esc -> 女 # 2$ 2 3 5 6 7 8 9. tab e r t y a f k ift V alt ctriarrow_forward
- If hybrid DNA is allowed to replicate for one generation in medium containing N15 and for second generation in medium containing N14, then what should be the proportion of light, heavy and hybrid DNA respectively? 1. 25%, 0% and 75% 2. 0%, 25% and 75% 3. 25%, 25% and 50% 4. 0%, 0% and 100%arrow_forwardWhich of the following statements is TRUE? Synthesis of the lagging strand requires more DNA helicase enzymes than synthesis of the leading strand. The lagging strand is synthesized in a semiconservative fashion while the leading strand is synthesized in a conservative fashion. The leading strand is replicated continuously for the entire length of the chromosome, e.g. while the lagging strand has multiple origins of replication The lagging strand has a higher mutation rate than the leading strand. None of the answers are true.arrow_forwardWhy is the company Qiagen has more refined DNA extraction steps than a normal Strawberry DNA extraction practical? Summary of Qiagen DNA extraction steps Add ATL buffer and grind with sample. Add 20 microliters of enzyme Proteinase K to degrade protein into a 1.5-2ml microcentrifuge tube. Add 200 microlitres AL lysis buffer, and mix by vortexing for 5–10 seconds, which breaks cell membrane allowing DNA to be released. Incubate the sample at 56 degrees for 10 minutes. Mix the cell lysate with 200 microlitres ethanol by pipetting it at the side of the microcentrifuge wall so DNA precipitates. The DNA forms a white layer and the remaining liquid is discarded. Pipet the mixture into DNeasy Mini spin column placed in a 2 ml collection tube. Centrifuge for a minute at 8000 rpm. Place the mini spin column into a 2 ml collection tube, add 500 µl Buffer AW1, and centrifuge for 1 min at 8000 rpm. Then add it to a new 2 ml collection tube (provided), add 500 µl Buffer AW1, and centrifuge for 1…arrow_forward
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