Your PhD thesis advisor has given you the task of preparing a human genomic DNA library.  a. How will you prepare DNA fragments from the human genomic DNA for use in construction of this library assuming that you want the insert sizes to be about 20000bp in size? b. What vector will you use for construction of this library? c. How will you ligate the genomic DNA fragments into the vector? d. What host will you use for propagation of your library? e. What probe would you use for screening your library assuming that you wanted to isolate the human insulin gene? f. How many clones would you have to screen to be pretty sure that you will indeed find a clone for insulin?

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Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.