The pLacZ cloning vector has an MCS with 10 different palindromes, that is, where you can clone using 10 different enzymes. How many different combinations of enzymes could be used to clone? (This is a measure of the versatility of the vector) a) 2^10 b. 4^3 c. 10^2 d. 3^4 14)One of the first vaccines achieved thanks to the tissue culture technique was that of influenza, True or False? 15) In the context of biotechnology, what does the term clone mean? a) Synonym of Dolly, identical copies of a multicellular organism b. Many copies of some cellular organelle. c. Production of drugs and medicines for diseases, but under the control of ethical standards d. Chimeric molecules (vector and insert), inside a bacteria replicating to form a colony e. Identical copies of proteins from recombinant DNA
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
13) The pLacZ cloning vector has an MCS with 10 different palindromes, that is, where you can clone using 10 different enzymes. How many different combinations of enzymes could be used to clone? (This is a measure of the versatility of the vector)
a) 2^10 b. 4^3 c. 10^2 d. 3^4
14)One of the first vaccines achieved thanks to the tissue culture technique was that of influenza, True or False?
15) In the context of
a) Synonym of Dolly, identical copies of a multicellular organism
b. Many copies of some cellular organelle.
c. Production of drugs and medicines for diseases, but under the control of ethical standards
d. Chimeric molecules (vector and insert), inside a bacteria replicating to form a colony
e. Identical copies of proteins from recombinant DNA
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