You have isolated a new endoglucanase enzyme. Through various enzymes assays, you are trying to determine the optimal conditions for this enzyme's activity. You make the following observations: i. when performing the assay at 40°C using buffers with different possibilities, you observe that the enzyme has no activity below pH 3 or above pH 11. You observe high activity at pH 6 and about 50% of this activity level at pH 5 and pH 7. ii. When performing the assay using a buffer at pH 6, you measure almost no activity at a temperature of 10°C. However, the activity steadily rises when tested at 20°C, 30°C, 40°C and 50°C, where the highest activity was measured, before declining at 60°C. At 70°C there is no measurable activity. Explain the meaning of these observations and identify the optimal pH and temperature conditions for the endoglucanase enzyme. Further more, explain why these observations occur based on your knowledge of the effects of pH and temperature on enzymes.
You have isolated a new endoglucanase enzyme. Through various enzymes assays, you are trying to determine the optimal conditions for this enzyme's activity. You make the following observations: i. when performing the assay at 40°C using buffers with different possibilities, you observe that the enzyme has no activity below pH 3 or above pH 11. You observe high activity at pH 6 and about 50% of this activity level at pH 5 and pH 7. ii. When performing the assay using a buffer at pH 6, you measure almost no activity at a temperature of 10°C. However, the activity steadily rises when tested at 20°C, 30°C, 40°C and 50°C, where the highest activity was measured, before declining at 60°C. At 70°C there is no measurable activity. Explain the meaning of these observations and identify the optimal pH and temperature conditions for the endoglucanase enzyme. Further more, explain why these observations occur based on your knowledge of the effects of pH and temperature on enzymes.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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You have isolated a new endoglucanase enzyme. Through various enzymes assays, you are trying to determine the optimal conditions for this enzyme's activity. You make the following observations:
i. when performing the assay at 40°C using buffers with different possibilities, you observe that the enzyme has no activity below pH 3 or above pH 11. You observe high activity at pH 6 and about 50% of this activity level at pH 5 and pH 7.
ii. When performing the assay using a buffer at pH 6, you measure almost no activity at a temperature of 10°C. However, the activity steadily rises when tested at 20°C, 30°C, 40°C and 50°C, where the highest activity was measured, before declining at 60°C. At 70°C there is no measurable activity.
Explain the meaning of these observations and identify the optimal pH and temperature conditions for the endoglucanase enzyme. Further more, explain why these observations occur based on your knowledge of the effects of pH and temperature on enzymes.
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