The purpose of the progress curve is to determine if an enzymatic reaction rate remains constant for given concentrations of enzyme and substrate, for a given assay time (20 min). How long did the rate of reaction of WGAP remain constant in your experiments? How do you know? What would cause the reaction rate to not be constant and plateau after some time?
The purpose of the progress curve is to determine if an enzymatic reaction rate remains constant for given concentrations of enzyme and substrate, for a given assay time (20 min). How long did the rate of reaction of WGAP remain constant in your experiments? How do you know? What would cause the reaction rate to not be constant and plateau after some time?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Question
The purpose of the progress curve is to determine if an enzymatic reaction rate remains constant for given concentrations of enzyme and substrate, for a given assay time (20 min). How long did the rate of reaction of WGAP remain constant in your experiments? How do you know? What would cause the reaction rate to not be constant and plateau after some time?
Transcribed Image Text:27
28 Part B: progr Abs
29
30
31
32
33
34
35
36
37
38
39
40
41
42
44
5
5
10
10
15
15
20
20
25
25
Unmol p-np
0.109 0.012868
0.133 0.015701
0.294 0.034708
0.29 0.034236
0.386 0.045569
0.329 0.03884
0.402 0.047458
0.487 0.057493
0.511 0.060326
0.596 0.070361
"Mur
amount of p-np (umoles)
0.08
0.07
0.06
0.05
0.04
0.03
0.02
0.01
0
0
5
10
15
Reaction time (min)
y=0.0027x
20
25
30
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