You wish to isolate an enzyme from its native source and need a method for measuring its activity throughout the purification. However, neither the substrate nor the product of the enzymecatalyzed reaction can be detected by spectroscopy. You discover that the product of the reaction is highly antigenic when injected into mice. Propose a strategy to develop a suitable assay for this enzyme.
Nucleotides
It is an organic molecule made up of three basic components- a nitrogenous base, phosphate,and pentose sugar. The nucleotides are important for metabolic reactions andthe formation of DNA (deoxyribonucleic acid) and RNA (ribonucleic acid).
Nucleic Acids
Nucleic acids are essential biomolecules present in prokaryotic and eukaryotic cells and viruses. They carry the genetic information for the synthesis of proteins and cellular replication. The nucleic acids are of two types: deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). The structure of all proteins and ultimately every biomolecule and cellular component is a product of information encoded in the sequence of nucleic acids. Parts of a DNA molecule containing the information needed to synthesize a protein or an RNA are genes. Nucleic acids can store and transmit genetic information from one generation to the next, fundamental to any life form.
You wish to isolate an enzyme from its native source and need a method for measuring its activity throughout the purification. However, neither the substrate nor the product of the enzymecatalyzed reaction can be detected by spectroscopy. You discover that the product of the reaction is highly antigenic when injected into mice. Propose a strategy to develop a suitable assay for this enzyme.
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