Homework Help is Here – Start Your Trial Now!
Science
Biology
The measurement of LDH in the serum can be used to diagnose whether tissue damage has occurred. Students were tasked with the development of an assay for LDH activity in human serum samples from a control patient (healthy) and a patient who had suffered cardiac damage. Two samples were provided, labelled C and D (Control and Diseased serum). The students decided to follow the loss of absorbance of NADH in an appropriate assay buffer containing pyruvate as the substrate. Q1 (a) By examining the absorbance spectra of both NAD*and NADH below, comment why this approach is suitable strategy. [3] -

The measurement of LDH in the serum can be used to diagnose whether tissue damage has occurred. Students were tasked with the development of an assay for LDH activity in human serum samples from a control patient (healthy) and a patient who had suffered cardiac damage. Two samples were provided, labelled C and D (Control and Diseased serum). The students decided to follow the loss of absorbance of NADH in an appropriate assay buffer containing pyruvate as the substrate. Q1 (a) By examining the absorbance spectra of both NAD*and NADH below, comment why this approach is suitable strategy. [3] -

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
See similar textbooks
icon
Related questions
Question
100%
The measurement of LDH in the serum can be used to diagnose whether tissue damage has occurred. Students were tasked with the development of an assay for LDH activity in human serum samples from a control patient (healthy) and a patient who had suffered cardiac damage. Two samples were provided, labelled C and D (Control and Diseased serum). The students decided to follow the loss of absorbance of NADH in an appropriate assay buffer containing pyruvate as the substrate. Q1 (a) By examining the absorbance spectra of both NAD*and NADH below, comment why this approach is suitable strategy. [3] 16 NAD* 12 NADH 240 280 320 360 Wavelength (nm) Q1 (b). What would be the optimum wavelength (Aopti) at which they should measure absorbance? Give a reason for your answer. [2] Molar absorbance x 10
Transcribed Image Text:The measurement of LDH in the serum can be used to diagnose whether tissue damage has occurred. Students were tasked with the development of an assay for LDH activity in human serum samples from a control patient (healthy) and a patient who had suffered cardiac damage. Two samples were provided, labelled C and D (Control and Diseased serum). The students decided to follow the loss of absorbance of NADH in an appropriate assay buffer containing pyruvate as the substrate. Q1 (a) By examining the absorbance spectra of both NAD*and NADH below, comment why this approach is suitable strategy. [3] 16 NAD* 12 NADH 240 280 320 360 Wavelength (nm) Q1 (b). What would be the optimum wavelength (Aopti) at which they should measure absorbance? Give a reason for your answer. [2] Molar absorbance x 10
Expert Solution
steps

Step by step

Solved in 2 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Knowledge Booster
Hematology
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education
SEE MORE TEXTBOOKS