Briefly discuss the effect of salt, pH and temperature on the action of salivary amylase. Then, list TWO possible errors in this experiment. Suggest how these errors could be prevented. LabelControlTestTemp testAcid testAlkaline testSalt testContent + Treatment1 mL starch solution + 1.5 mL H₂O1 mL starch solution + 0.5 mL enzyme + 1 mL H₂O1 mL starch solution + 0.5 mL enzyme + 1 mL H₂O;Keep in 75°C water-bath1 mL starch solution + 0.5 mL enzyme + 1 mL 0.5M HCl;Incubate at 37°C1 mL starch solution + 0.5 mL enzyme + 1 mL 0.5M NaOH;Incubate at 37°C1 mL starch solution + 0.5 mL enzyme + 1 mL 1M NaCl;Incubate at 37°C

Salivary amylase breaks down complex carbohydrates into smaller pieces. The activity of salivary…

Answered: Briefly discuss the effect of salt, pH…

Briefly discuss the effect of salt, pH and temperature on the action of salivary amylase. Then, list TWO possible errors in this experiment. Suggest how these errors could be prevented.

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

See similar textbooks

Similar questions

Hexokinase is an enzyme that uses glucose as its substrate. the rate at which hexokinase breaks down glucose is the same when there is a lot of glucose in the cell compared to when there is only a small amount of glucose in the cell A) TRUEB)FALSE
The following kinetic data were collected for prostaglandin endoperoxide synthase, an enzyme involved in pain and inflammation. Using data in the first two columns, use a graphical analysis to determine the Vmax and the Km of the enzyme. Ibuprofen (Advil) is an inhibitor of prostaglandin endoperoxide synthase. By inhibiting this enzyme, ibuprofen helps to reduce pain and fever. Using the data in the first and third columns, determine the type of inhibition that ibuprofen exerts on prostaglandin endoperoxide synthase. In the presence of the inhibitor, determine the Vmax and the Km of the enzyme. [S] mM Product formed Product formed (mmol/min) with 10 mg/mL ibuprofen mmol/min) 0.5 23.5 16.67 1.0 32.2 25.25 1.5 36.9 30.49 2.5 41.8 37.04 3.5 44.0 38.91
Why is derivatization (i.e., transesterification) of triglycerides or lipids to fatty acid methyl esters needed for the GC/MS experiment?
In the following scenarios, glucose is absent. Judging these systems as a whole, decide if B-galactosidase and permease are constitutive (always), unable (never), or inducible for each situation. Note, you must use the words inducible, unable, or constitutive. You should only have one answer per block. Please format your answer as a table and upload as a pdf or doc file B-galactosidase Permease |A) I POZYA I PΟΖΥΤΑ" B-galactosidase Permease B) IS ΡΟΖΥΤΑ I POCZYA B-galactosidase Permease С) I POZ'YA ΓΡΟΖΥΑ B-galactosidase Permease D) I PO̟ZYA ΡΟΖΥΤΑ B-galactosidase Permease E) I PΟΖΥΑ I POZ'YA
HbA1c is a glycated hemoglobin in which a glucose molecule is covalently bound to the N-terminal valine of a hemoglobin subunit. True False
Answer the following questions about the inhibition of acetylcholine esterase by neostigmine. 5a. Does neostigmine have a structure that enables it to bind reversibly to the ACbE active site? 5b. Is neostigmine catalytically processed by ACHE? 5c. How does the stability of the carbamolyl-enzyme intermediate produced by treatment of ACbE with neostigmine compare to the stability of the acetyl- enzyme intermediate produce when ACb, is hydrolyzed?
a) Use your sketching and writing skills to explain the relationship between metabolic rate (M) and body weight (W): M = aWb b) Based on your answer to question a), generate a new graph to explain how the exponent b in the allometric relationship changes between different groups of animals; and what would happen graphically if M is expressed per gram of body weight: M/W =aW(b-1)
Write the sequence of steps for the biosynthesis of phosphatidylcholine by the salvage pathway from oleate, palmitate, dihydroxyacetone phosphate, and choline. Name the enzyme for each step.
Patients with a form of early-onset diabetes were found to carry a variety of mutations reducing the function of the glucokinase (GK) enzyme. These patients are heterozygous for one of the mutations, and thus show only partial rather than complete loss of GK activity. Answer the following questions about these patients. 1. How would blood sugar levels likely be affected in GK-mutation patients? Briefly explain your choice (25 words or less) a. Higher than normal b. Normal C. Lower than normal d. Cannot predict based on the provided information
Answer the following using the picture attached: A. How does pepsin differ from chymotrypsin in terms of its active site residues and mechanism? B. How do these changes permit pepsin to operate at such a low pH?
Transport of glucose across the basolateral membrane of intestinal epithelial cells and into the vascular system requires which of the following conditions to occur? Select one: a. Sodium concentration on the inside of the epithelial cell is equal to that outside the basolateral membrane b. Sodium concentration is higher on the inside of the epithelial cell than on the outside of the basolateral membrane c. Sodium concentration inside the epithelial cell is lower than sodium concentration in the intestinal lumen d. Sodium concentration inside the epithelial cell is higher than that sodium concentration in the intestinal lumen
One such neurotoxin, Sarin (chemical name: propan-2-yl methylphosphonofluoridate) is considered a weapon of mass destruction by the United Nations by virtue of its ability to kill with great efficiency in very low concentrations. a. Where does Sarin have its effect (what is/are its target) and describe the mechanism of action of Sarin (how does it kill)? b. Describe the physical symptoms observed in those stricken with Sarin that are consistent with this mechanism of action. Include flaccid/tonic paralysis, as applicable.