Briefly discuss the effect of salt, pH and temperature on the action of salivary amylase. Then, list TWO possible errors in this experiment. Suggest how these errors could be prevented.
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Briefly discuss the effect of salt, pH and temperature on the action of salivary amylase. Then, list TWO possible errors in this experiment. Suggest how these errors could be prevented.
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- Test Description Terminal Terminal Medium, Incubation Incubation Results temperature time (°C) electron enzyme reagent ассeptor 1 Oxidase test Tryptic 24 – 48 hours soy agar (TSA), QxiDrop 2 Catalase Охудen test N/A Nitrate 37 reductase N/A testSerial dilution = previous dilution x dilution 11 mL [2] 3 mL Colony count: 100 0.1 mL sample [1] 9.9 mL 5 mL 0.1 ml. Cokony count Dilution: 10-1 10-3 [3] 150 Dilution factor (DF): [4] [5] [6] Compute for the CFU/mL of the sample. Show your solution and express your final answer to 2 significant figures.Sulfur Indole Motility (SIM) Medium H2S produced, color and +/-: ______________________________ Indole present/Tryptophan hydrolysis, color and +/-: ___________________________ Motile or non-motile: _____________________________
- Time point (min) Absorbance of culture at 660nm Approximate cell concentration Approximate # cells in 1mL extract 0 0.298 1.49 x 108 cells/mL 1.49 x 108 cells 10 0.316 1.58 x 108 cells/mL 1.58 x 108 cells 20 0.374 1.87 x 108 cells/mL 1.87 x 108 cells 30 0.429 2.145 x 108 cells/mL 2.145 x 108 cells 40 0.512 2.56 x 108 cells/mL 2.56 x 108 cells 50 0.544 2.72 x 108 cells/mL 2.72 x 108 cells 60 0.607 3.035 x 108 cells/mL 3.035 x 108 cells a. Using these data, prepare a growth curve of this strain ofEscherichia coli (E. coli).b. Estimate the doubling time for this strain of E. Coli. Clearly showhow you estimated this value from the empirical data presented.MOLLISCH TEST You can use this as your reference : https://youtu.be/rKng5-ij6kQWhat does the phrase "pipetting up and down" mean and why is this technique used? The phrase "pipetting up & down" means to triturate. Th technique is used to mix (or homogenize) a solutic 7 On what part of a microcentrifuge tube should you write a label? Describe the order in which you filled the tubes in Step 2 of Procedure 4. Did this order result in maximum efficiency? If not, what order would be most efficient? 9 What does the phrase "spinning down" mean and why is this technique used? The word 'spinning down" means, To diminish in energy i to slow down o out; to be gradually ended or concelled. It is used to reduce its spin speed from that required for reading and writing. 10 Why was the comb placed in the middle rather than at one end of the gel for this electrophoresis experime- The comb is in the center of the gel Since the dyes used have positive or negative charges and can therefore migrate in differen
- Imagine you have been given a liquid culture of yeast with a starting concentration of 3.67 x 10' cells/ml and are asked to carry out the sample dilution process shown in the figure below. 100μl 100μl 100μl 100μl 100μl 0.9ml 0.9ml 0.9ml H2O H₂O 6.9ml 0.9ml H₂O H₂O H₂O Original 10-1 102 10-3 104 Culture 105 100μl 100μl 100μl Plate A Plate B Plate C a. How many colonies should have been present on Plate A in this example? - Answers must be whole numbers as partial colonies are not expected. b. Imagine you carried out the same dilution scheme shown in the figure above, but now, you do not know the concentration of the original culture. If you counted 163 colonies on Plate B, what is the concentration of cells/ml in the original culture?This is the growth curve for Clostirdium (incubated under optimal growth conditions). Use it to answer the following questions. 3 Cell concentration (million cells/ml) 2.5 2 0.2 1.5 1 0.5 0 2 The growth rate is lag log 0.25 4 0.32 0.5 units 6 million of cells per ml (Image description.: Horizontal axis is time in hours, Vertical is cell concentration in millions of cells per ml. Curve starts at 0,0, increases by 4 hours to 0.5 million cells/ml, goes up steeply over two hours from 0.5 million/ml at 4 hours to 2.5 million cells/ml at 8 hours, 8-12 hour shows an increase of 0.3 to 2,8 million cellls/ml and last 2 hours is flat at 2.8million cells/ml.) From 12 hr to 14 hr is called phase. lunar 2 8 Time (hours) 10 5 stationary 12 cells 14 death 16 million of cells per ml per hour hours million of cellsDetermine what percentage of the culture was living (viable) and what percentage was dead (mortality). Plates Plate Dilution Volume plated No.of colonies Avg No Concentration of diluted sample Cd(cells/mL) Concentration of original sample Cu(cells/mL) 1 10^-3 10ul R1=130,R2= 110,R3=210 150 150mL 1.50*10^6 Volume of cells(mL) Volume of diluent(mL) Total dilution(D) Hemocytometer count Avg cells in 1 mm^2 area Concentration of diluted sample Cd(cells/mL) Concentration of original sample Cu(cells/mL) 4.3 0.5 0.1 grid 1= 171 , grid2 = 185 178 1.78*10^5 1.78*10^6
- Which of the following is the a-anomer? H но H Но H2C H ОН H2C- нн ОН со 4 1 2 1 Submit 3 -OH Н ОН -OH ОН Н there is no a-anomer ОН ОН Н Request Answer H Но НО Н H2C нн ОН 2 H2C ОН -OH 4 Н ОН н н H OH -О. ОН ОН Н ОН НA culture is incubated for 10 hours. 1 hours after inoculation it reached the exponential growth phase. At this time point the cell density was 1x10^4 cells/ml. 5 hours after inoculation (still during the exponential growth phase) the cell density was 1x10^7 cells. Calculate K and g(t). The growth constant (k) is minute. (round to 3 decimal points) The generation time (gt) is minutes. (round to whole number)By alkaloidal reagent Place 3mL of egg albumin solution in each of the two test tubes, to one tube add 1 – 2 ml of tannic acid solution and to other one picric acid. Describe the appearance of the protein solutions in each of the tubes after addition of the alkaloidal reagents.