Briefly discuss the effect of salt, pH and temperature on the action of salivary amylase. Then, list TWO possible errors in this experiment. Suggest how these errors could be prevented. LabelControlTestTemp testAcid testAlkaline testSalt testContent + Treatment1 mL starch solution + 1.5 mL H₂O1 mL starch solution + 0.5 mL enzyme + 1 mL H₂O1 mL starch solution + 0.5 mL enzyme + 1 mL H₂O;Keep in 75°C water-bath1 mL starch solution + 0.5 mL enzyme + 1 mL 0.5M HCl;Incubate at 37°C1 mL starch solution + 0.5 mL enzyme + 1 mL 0.5M NaOH;Incubate at 37°C1 mL starch solution + 0.5 mL enzyme + 1 mL 1M NaCl;Incubate at 37°C

Salivary amylase breaks down complex carbohydrates into smaller pieces. The activity of salivary…

Answered: Briefly discuss the effect of salt, pH…

Briefly discuss the effect of salt, pH and temperature on the action of salivary amylase. Then, list TWO possible errors in this experiment. Suggest how these errors could be prevented.

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question

Briefly discuss the effect of salt, pH and temperature on the action of salivary amylase. Then, list TWO possible errors in this experiment. Suggest how these errors could be prevented.

Label
Control
Test
Temp test
Acid test
Alkaline test
Salt test
Content + Treatment
1 mL starch solution + 1.5 mL H₂O
1 mL starch solution + 0.5 mL enzyme + 1 mL H₂O
1 mL starch solution + 0.5 mL enzyme + 1 mL H₂O;
Keep in 75°C water-bath
1 mL starch solution + 0.5 mL enzyme + 1 mL 0.5M HCl;
Incubate at 37°C
1 mL starch solution + 0.5 mL enzyme + 1 mL 0.5M NaOH;
Incubate at 37°C
1 mL starch solution + 0.5 mL enzyme + 1 mL 1M NaCl;
Incubate at 37°C

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