The following experiment was set up to understand how catechol oxidase converts catechol oxidase catechol into the brown product called benzoquinone and how it can be inhibited.  Answer the questions based on the following tube contents and results: Tube 1: 0 ml catechol, 0.5 ml Potato extract, 2.5ml buffer, 0.5 ml inhibitor.  After observation there was no color development.  Tube 2: 0.5 ml catechol, 0.5 ml Potato extract, 2 ml buffer, 0.5 ml inhibitor.  After observation there was no color development.  Tube 3: 1 ml catechol, 0.5 ml Potato extract, 1.5 ml buffer, 0.5 ml inhibitor.  After observation the solution turned brown. Tube 4: 2 ml catechol, 0.5 ml Potato extract, 0.5 ml buffer, 0.5 ml inhibitor.  After observation the solution turned brown.   Which tube (1, 2, 3, or 4) was the control for this experiment?  Which experimental component was the substrate  Which experimental combonent contained the enzyme  Based on these results was the inibhitor a competative inhibitor or noncompetative inhibtor?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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The following experiment was set up to understand how catechol oxidase converts catechol oxidase catechol into the brown product called benzoquinone and how it can be inhibited.  Answer the questions based on the following tube contents and results:

Tube 1: 0 ml catechol, 0.5 ml Potato extract, 2.5ml buffer, 0.5 ml inhibitor.  After observation there was no color development. 

Tube 2: 0.5 ml catechol, 0.5 ml Potato extract, 2 ml buffer, 0.5 ml inhibitor.  After observation there was no color development. 

Tube 3: 1 ml catechol, 0.5 ml Potato extract, 1.5 ml buffer, 0.5 ml inhibitor.  After observation the solution turned brown.

Tube 4: 2 ml catechol, 0.5 ml Potato extract, 0.5 ml buffer, 0.5 ml inhibitor.  After observation the solution turned brown.

 

Which tube (1, 2, 3, or 4) was the control for this experiment? 

Which experimental component was the substrate 

Which experimental combonent contained the enzyme 

Based on these results was the inibhitor a competative inhibitor or noncompetative inhibtor? 

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