A mixture of dipeptides consisting of Lys-Gly, Thr-His, Ser-Leu, Glu-Gln wasapplied to column packed with cation-exchange resin at pH 6.0. Which ofthese dipeptides would be eluted from the column first?O Lys-Gly firstO Thr-His firstO Ser-Leu firstO Glu-Gln firstOA mixture of Thr-His and Ser-Leu first

Ion-exchange chromatography is a type of protein purification technique in which proteins are…

Answered: A mixture of dipeptides consisting of…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

See similar textbooks

Similar questions

Prepare the following LB media with antibiotic added. A 100ml of LB media with 25 micro/ml of Amp and 100micro/ml of Kan final concentration. A 100ml of LB is provided and amp of Kan stock at 100 mg/ml and 50 mg/ml are also provided. Find how much each antibiotic stock solution is needed to add 100ml of LB to reach desired antibiotic concentration.
Given this, if you used 6g of vitamin Z powder to make 20 ml of solution, what is the % concentration of this solution? (I gave the image since I don't know if that info is needed to solve this question.)It also gives a follow-up, if you can help here too: You work in a lab as a summer student. One of your tasks is to make sure that there is enough cell culture medium containing antibiotics to grow bacteria. One day you realize that there is only 5 ml of 10% Antibiotic stock solution in the freezer. You decide to use it all to prepare the working culture medium with 0.01% antibiotic. In the lab there is plenty of growth medium without antibiotics. (Note: dilution in medium is like dilution in water). You remember the equation to make dilutions of stock solutions. You usually use this formula to calculate the required volume of a stock solution, but you realize it can apply here as well, even though the unknown is the final volume. So, you make that dilution. Given that each bacterial…
What is the oligodynamic effect? To which chemicals does the term oligodynamic effect apply? Give 2 examples of antiseptics based upon the oligodynamic effect.
100ml of LB media with 25 μg/ml of Amp and 100 μg/ml of Kan final concentration. You have 100ml of LB provided and Amp and Kan stocks at 100 mg/ml and 50 mg/ml provided. Determine how much of each antibiotic stock solution you need to add to 100ml of LB to reach desired antibiotic concentration.
&. How many milldiners of a 2wv Lidocaine HCI stock selution in water should be used in preparing 30 ml of a 0.5% wiv Lidocaine HCI solution? . From the following formula for antificial tears, caleulate the quantity of each ingrodient roquired to prepare half donen of the 0ml containers. Poyviny alcohol 149 Povidone Chiorobutanol Serile sodum chioride soution a.%. s to 100 m How many milldiners of water should be added to 60 ml. of a 2% wiv agqueous solution to prepare a 1% wiv solution?
What of the following is correct regarding the deoxy sugar shown below? CH3 HO НО ОН It is a D-sugar O It is an L-sugar O It is the alpha anomer It is the beta anomer
essential oils have been studied as potential antimicrobial agents. These naturally occuring compounds are linked to having the following type of activity in clinical trials: virucidal, septicidal, bactricidal, fungicidal
What type of ion exchange resin and what buffer pH would you use to purify pepsin from an extract of pig cells? (Assume that pepsin should bind to the column resin). Anion exchanger at pH less than 3 Cation exchanger at pH greater than 6 Cation exchanger at pH 6 Anion exchanger at pH greater than 3
A patient presents with a large infected abscess, and is prescribed a 100.0 mg IV treatment of Micafungin, given daily for 2 weeks. The Micafungin is available in 50.0 mg vials. How many vials are necessary for this course of treatment?
You are studying the tripep de Lys-Val-Thr. a) Could you use the absorbance at 280 nm to determine the concentra on of this tripep de? Briefly explain your answer. b) Show how the charge of this pep de would change at different pHs.
You resuspend 1 ml of of a purified chlorophyll sample in 10 ml of 80% acetone, and determine that the optical density of the solution (at 652 nm) is 0.88. What is the concentration of your original preparation?
Select the product for the following cyclization CHO OH OH OH H OH OH Но HO HO OH но Но - OH HO HO Но. OH H- OH OH H H H ČH2OH 1 3 О 1&2 1 2

SEE MORE QUESTIONS

Recommended textbooks for you

Biochemistry

ISBN:

9781319114671

Author:

Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:

W. H. Freeman

Lehninger Principles of Biochemistry

Biochemistry

ISBN:

9781464126116

Author:

David L. Nelson, Michael M. Cox

Publisher:

W. H. Freeman

Fundamentals of Biochemistry: Life at the Molecul…

Biochemistry

ISBN:

9781118918401

Author:

Donald Voet, Judith G. Voet, Charlotte W. Pratt

Publisher:

WILEY

Biochemistry

ISBN:

9781319114671

Author:

Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:

W. H. Freeman

Lehninger Principles of Biochemistry

Biochemistry

ISBN:

9781464126116

Author:

David L. Nelson, Michael M. Cox

Publisher:

W. H. Freeman

Fundamentals of Biochemistry: Life at the Molecul…

Biochemistry

ISBN:

9781118918401

Author:

Donald Voet, Judith G. Voet, Charlotte W. Pratt

Publisher:

WILEY

Biochemistry

ISBN:

9781305961135

Author:

Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal

Publisher:

Cengage Learning

Biochemistry

ISBN:

9781305577206

Author:

Reginald H. Garrett, Charles M. Grisham

Publisher:

Cengage Learning

Fundamentals of General, Organic, and Biological …

Biochemistry

ISBN:

9780134015187

Author:

John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson

Publisher:

PEARSON

SEE MORE TEXTBOOKS