1. Suppose the following kinetic data was collected for an enzymatic reaction For each substrate concentration, calculate and show the average initial rate and its standard deviation. [substrate), mM initial rate, min¹ Average initial rate, min 150 62 150 60 150 63 40 44 40 41 4222 40 43 12 11 15 Calculation of rates (min) for acid phosphatase reaction General Procedure for the End Point Assay of What Germ Acid Phosphate The general procedure used for each assay except the concentration of p-nitrophenyl phosphate was varied. The concentration of acid phosphatase was 7.2 μM) and each assay involve a 5-minute incubations with enzyme. Using this information along with the extinction coefficient of the p- nitrophenol product of the reaction allows for the initial rate to be expressed in terms of min¹. Vo [E] = absorbance at 410 nm 0.677 = initial rate in min-1
1. Suppose the following kinetic data was collected for an enzymatic reaction For each substrate concentration, calculate and show the average initial rate and its standard deviation. [substrate), mM initial rate, min¹ Average initial rate, min 150 62 150 60 150 63 40 44 40 41 4222 40 43 12 11 15 Calculation of rates (min) for acid phosphatase reaction General Procedure for the End Point Assay of What Germ Acid Phosphate The general procedure used for each assay except the concentration of p-nitrophenyl phosphate was varied. The concentration of acid phosphatase was 7.2 μM) and each assay involve a 5-minute incubations with enzyme. Using this information along with the extinction coefficient of the p- nitrophenol product of the reaction allows for the initial rate to be expressed in terms of min¹. Vo [E] = absorbance at 410 nm 0.677 = initial rate in min-1
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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![1. Suppose the following kinetic data was collected for an enzymatic reaction For each
substrate concentration, calculate and show the average initial rate and its standard
deviation.
[substrate), mM
initial rate, min¹
Average initial rate, min¹
150
62
150
60
150
63
40
44
40
41
40
43
222
12
11
15
Calculation of rates (min) for acid phosphatase reaction
General Procedure for the End Point Assay of What Germ Acid Phosphate
The general procedure used for each assay except the concentration of p-nitrophenyl phosphate
was varied. The concentration of acid phosphatase was 7.2 μM) and each assay involve a 5-minute
incubations with enzyme. Using this information along with the extinction coefficient of the p-
nitrophenol product of the reaction allows for the initial rate to be expressed in terms of min¹.
Vo
[E]
=
absorbance at 410 nm
0.677
= initial rate in min-1](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fc3db2bc1-178c-4c17-9613-575b914b3af7%2F7b14e05b-b240-4752-b36a-39616dff4923%2Fqhlyfb_processed.png&w=3840&q=75)
Transcribed Image Text:1. Suppose the following kinetic data was collected for an enzymatic reaction For each
substrate concentration, calculate and show the average initial rate and its standard
deviation.
[substrate), mM
initial rate, min¹
Average initial rate, min¹
150
62
150
60
150
63
40
44
40
41
40
43
222
12
11
15
Calculation of rates (min) for acid phosphatase reaction
General Procedure for the End Point Assay of What Germ Acid Phosphate
The general procedure used for each assay except the concentration of p-nitrophenyl phosphate
was varied. The concentration of acid phosphatase was 7.2 μM) and each assay involve a 5-minute
incubations with enzyme. Using this information along with the extinction coefficient of the p-
nitrophenol product of the reaction allows for the initial rate to be expressed in terms of min¹.
Vo
[E]
=
absorbance at 410 nm
0.677
= initial rate in min-1
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