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C. endpoints (values of the dependent variable, or data) should be plotted on the vertical or Y axis. Questions 1. What is the pH optimum for this exercise? (At what pH is the rate maximum?) pH of 10.

C. endpoints (values of the dependent variable, or data) should be plotted on the vertical or Y axis. Questions 1. What is the pH optimum for this exercise? (At what pH is the rate maximum?) pH of 10.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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C. endpoints (values of the dependent variable, or data) should be plotted on the vertical or Y axis. Questions 1. What is the pH optimum for this exercise? (At what pH is the rate maximum?) 2. Based on your data, predict what the endpoint would be at a pH of 10. 3. What is the effect on the endpoint of raising or lowering the pH above or below the pH with the lowest endpoint? 4. During the exercise, what variables are held constant? What variables are manipulated? What variables are measured? Held constant? Manipulated? Measured?
Transcribed Image Text:C. endpoints (values of the dependent variable, or data) should be plotted on the vertical or Y axis. Questions 1. What is the pH optimum for this exercise? (At what pH is the rate maximum?) 2. Based on your data, predict what the endpoint would be at a pH of 10. 3. What is the effect on the endpoint of raising or lowering the pH above or below the pH with the lowest endpoint? 4. During the exercise, what variables are held constant? What variables are manipulated? What variables are measured? Held constant? Manipulated? Measured?
C. EFFECT OF PH ON THE RATE OF ENZYME REACTION a. Procedure 1. Choose a concentration of amylase that had an endpoint time of 60 to 90 seconds in Part A. This will probably be either 1.0 or 2.0 mg/ml. Only one amylase concentration will be used for this exercise. Pipette exactly 1 ml of this amylase concentration into each of 7 different test tubes. 2. Obtain 7 clean test tubes and add 2 ml of starch solution to each. Then add to 2 ml of one different pH buffer to each. You now have 7 identical test tubes with amylase and 7 different test tubes with starch and different buffers. 3. Place the two sets of tubes next to each other into a rack and immerse the entire rack in the 37°C water bath at your table. Allow about 10 minutes for the solutions in the tubes to reach the temperature of the bath. 4. Follow the timing procedure in part A and determine the endpoint times for each pair. To conserve time, if endpoint has not been reached in 500 seconds, STOP, and record the end time as 500+ seconds. **Remember to keep tubes in the heated bath water and return the contents of the dropper after each drop, so that the temperature will not change during the experiment. 5. Record all data and answer questions below. b. Results 1. Record your data below. pH 3.0 4.0 S.D 7.0 8.0 D Endpoint time in Seconds 500+ 160 90 90 100 390 560 2. Plot a graph comparing the pH with the endpoint. The values of the independent variable (pH values) should be plotted on the horizontal or X axis of the graph. The 61
Transcribed Image Text:C. EFFECT OF PH ON THE RATE OF ENZYME REACTION a. Procedure 1. Choose a concentration of amylase that had an endpoint time of 60 to 90 seconds in Part A. This will probably be either 1.0 or 2.0 mg/ml. Only one amylase concentration will be used for this exercise. Pipette exactly 1 ml of this amylase concentration into each of 7 different test tubes. 2. Obtain 7 clean test tubes and add 2 ml of starch solution to each. Then add to 2 ml of one different pH buffer to each. You now have 7 identical test tubes with amylase and 7 different test tubes with starch and different buffers. 3. Place the two sets of tubes next to each other into a rack and immerse the entire rack in the 37°C water bath at your table. Allow about 10 minutes for the solutions in the tubes to reach the temperature of the bath. 4. Follow the timing procedure in part A and determine the endpoint times for each pair. To conserve time, if endpoint has not been reached in 500 seconds, STOP, and record the end time as 500+ seconds. **Remember to keep tubes in the heated bath water and return the contents of the dropper after each drop, so that the temperature will not change during the experiment. 5. Record all data and answer questions below. b. Results 1. Record your data below. pH 3.0 4.0 S.D 7.0 8.0 D Endpoint time in Seconds 500+ 160 90 90 100 390 560 2. Plot a graph comparing the pH with the endpoint. The values of the independent variable (pH values) should be plotted on the horizontal or X axis of the graph. The 61
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