An extract of rat liver was prepared by homogenizing 65 g of tissue in 55 mL of buffer (Fraction 1). This extract was assayed for Enzyme X activity. The total activity of Enzyme X in Fraction 1 was 5,650 units and its specific activity was 11.5 units/mg. Fractionl was then passed down an ion-exchange column and the bound proteins were eluted using increasing concentrations of NaCl. The fractions from the column were assayed for activity of Enzyme X. The fractions containing activity were pooled to give a total volume of 6.5 mL (Fraction 2) The total activity of Enzyme X in Fraction 2 was 4,162 units and the specific activity was 75.5 units/mg. a) What is the % Yield of the ion exchange step? b) What is the Fold purification of the ion exchange step?

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Nutrient Growth Medium

It is a culture medium or nutrient broth for all kinds of microorganisms to grow properly and create their colony. This matrix provides support to increase the population of microbes through the method of cell proliferation. It is regarded as the food material for those organisms. It is a type of in vitro mechanism, in which the development of life is processed outside the host body. It can be solid, semi-solid, or liquid in nature. Different kinds of cells develop in different types of media.