I mutated carboxylic anhydrase enzyme active site residue. For example, Histidine 64 and valine 121. How would you test the function of the mutants?

Q: The macrolide below is a bacteria produced toxin. The primer was acetyl-Co-A. Three possible…

A: Enter Your Answer xA

Q: The image below shows the urea cycle. Based on the information in the image, which of the following…

A: Urea cycle is an important pathway to eliminate ammonia by converting it into urea which can easily…

Q: Outline the key strategy used to reduce the sensitivity of the beta-lactam ring of a penicillin to…

A: Penicillin is a β-lactam antibiotic extracted from a fungus or mold called penicillium and is used…

Q: You and your lab partners decide to redo the B-galactosidase lab. You prepare three test tubes:…

A: The correct volume to use in the calculation of units of enzyme activity is 250 uL. This volume…

Q: How are ribosomes affected in treacher Collins Syndrome disorder? Please write it in a…

A: Treacher Collins syndrome (TCS) is a genetic disorder which is characterized by deformities of the…

Q: Provide a stepwise, arrow-pushing mechanism for the following transformation. You may use general…

A: The reaction in question involves a lysis reaction catalyzed by a lyase enzyme using the coenzyme…

Q: Rabbits were in injected with [32 P] photsphate intraperitoneally ans asacrificed after 2, 5, 8, 12,…

A: This experiment examined the process of radioisotope labeling in rabbits. Rabbits were injected with…

Q: What is Folate Trap ? Explain the importance of Folate Trap ?

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Q: In bacteria which ribozyme catalyzes synthesis of peptide bonds between amino acids? 16S rRNA 5S…

A: the translation is the process by which the messenger rna is converted into polypeptide .

Q: 1) What is wrong with the nucleoside shown below? Focus on the sugar portion. What sugar should be…

A: We have two types of nucleic acids namely:DNA or Deoxyribonucleic acid andRNA or Ribonucleic acid…

Q: The formation of deoxyribonucleotide from ribonucleotide requires all the following except:…

A: A nucleotide is the basic part of nucleic acid that contains nitrogenous bases (adenine, thymine,…

Q: The nucleotides were chemically modified. Select the modification that will result in a nucleotide…

A: Nucleotides are of two types, ribonucleotides and deoxyribonucleotides. Ribonucleotides are composed…

Q: Predict the locations of 14C in orotate isolated from cells grown on a small amount of uniformly…

A: Introduction  All living things are composed primarily of cells. There are many trillions of cells…

Q: Explain why bacteria with the gene for the enzyme beta-lactamase are not susceptible to penicillin…

A: A beta-lactam ring is a four-membered lactam a cyclic amide, and beta-lactams are named so because…

Q: Based on the diagram, what can you conclude about how the chloramphenicol behaves as an antibiotic?

A: Chloramphenicol is a broad-spectrum antibiotic whose spectrum includes several gram-positive and…

Q: C) We looked at three serine protease enzymes: elastase, chymotrypsin, and trypsin. All three…

A: The enzyme which helps in the digestion of elastin and release by the pancreas is called elastase.…

Q: 7). You analyze a biosynthetic pathway in an unknown strain of bacteria. Given the following data…

A: 3-phosphoglycerate (PGA) is used by bacteria as the starting material to synthesize serine. From…

Q: The figure below shows the structures of the blood group antigens O, A and B.List all nucleotide…

A: The ABO blood group is an example of multiple alleles in which total three alleles are responsible…

Q: why is it nessecary to modify the RNA molecule?

A: RNA molecules are highly interesting molecules, which perform a variety of functions and helps in…

Q: at punctures the bacterial cell wall has just been recently isolated from the F the peptide reveals…

A: Trypsin cleave on the carboxy side of arginine and lysine residues. Chymotrypsin cleave on the…

Q: 2B. S. aureus hemolysin B attacks the RBC cell membrane by hydrolyzing the sphingomyelin headgroup:…

A: Sphingomyelin is a phospholipid with sphingosine  as platform molecule and phosphocholine as head…

Q: 1. Lets start by determining the products we would get from treating a polypeptide with some of the…

A: A peptide is a short chain of amino acid residues linked together via peptide bonds. The peptide…

Q: Radioactive uracil can be used to label all the pyrimidine residues in DNA. Explain how this occurs,…

A: Uracil is energetically less expensive to produce than thymine, which may account for its use in…

Q: Which of the following deoxyoligonucleotides will hybridize with the following sequence 5' AGACTGGTC…

A: Deoxyoligonucleotides are short strands of DNA that are composed of a sequence of…

Nitrogen metabolism

Nitrogen metabolism is the synthesis, utilization or uptake, breakdown, and excretion of nitrogenous compounds in the body. The nitrogen metabolism encompasses nitrogen fixation, biosynthesis, and the breakdown of amino acids, purines, and pyrimidine. Since nitrogen-containing compounds are required for various life processes, nitrogen is an important element of life.