Based on your Lineweaver-Burk plot, what is the mode of PaESBL-1 inhibition by NSAMB?What is the equation for the double-reciprocal plot at 20 nMnM NAMB? You contact an academic lab that specializes in synthesizing boronic acid analogs that have been shown to act as reversible inhibitors of the AmpC B-lactamase from Escherichia coll. The lab agrees to send you several of its compounds to test against PAESBL-1. You begin bylooking at the potential of (napthylmethanesulfonylamino)methaneboronic acid (NSAMB) to inhibit the P. aeruginosa B-lactamase:онBO=S- NHOHNSAMBYou perform a classic reversible inhibition experiment, where you measure the initial velocity for a range of substrate and inhibitor concentrations. For this assay, you use the artificial chromogenic substrate, CENTA:`NOCENTACENTA is hydrolyzed by B-lactamases to yield a product with absorbance at 405 nm.The results of your experiment are listed in the table:Initial velocity measurements (mAbs/s)CENTA concentration(µM)[NSAMB) = 0 nM[NSAMB) = 20 nM[NSAMB] = 40 nM[NSAMB) = 80 nM150.1250.1020.0870.066300.1670.1450.1290.105600.2000.1840.1700.1481200.2220.2120.2020.1862400.2350.2290.2240.213

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Answered: Based on your Lineweaver-Burk plot,…

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Biochemistry

Based on your Lineweaver-Burk plot, what is the mode of PaESBL-1 inhibition by NSAMB?

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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