Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter7: Basic Clinical Microbiology
Section7.7: Bacterial Identification And Antibiotic Susceptibility Testing
Problem 11RQ
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Question
Quantitatively describe the unique focusing capability of IEF and identify factors that increase resolution of proteins in IEF.
Expert Solution
Step 1
IEF stands for isoelectric focusing which is a technique used to resolve a mixture of proteins on a gel. It depends upon the isoelectric points (pI) of different proteins. A combination of IEF with SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) known as 2D (dimensional) gel electrophoresis is used widely to perform protein separation.
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