Principles Of Radiographic Imaging: An Art And A Science
6th Edition
ISBN:9781337711067
Author:Richard R. Carlton, Arlene M. Adler, Vesna Balac
Publisher:Richard R. Carlton, Arlene M. Adler, Vesna Balac
Chapter36: Bone Densitometry
Section: Chapter Questions
Problem 11RQ
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Question
Quantitatively describe the unique focusing capability of IEF and identify factors that increase resolution of proteins in IEF.
Expert Solution
Step 1
IEF stands for isoelectric focusing which is a technique used to resolve a mixture of proteins on a gel. It depends upon the isoelectric points (pI) of different proteins. A combination of IEF with SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) known as 2D (dimensional) gel electrophoresis is used widely to perform protein separation.
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