A hexapeptide of unknown sequence was isolated from an amphibian skin then sequenced.The following were the results of the sequencing process:A. When the peptide was treated with dinitrofluorobenzene (DNFB), DNP-ala and a mixture ofamino acids were produced.B. When the same intact peptide was treated with trypsin, a tetrapeptide of composition arg,met, ala and ser and a dipeptide were released.C. Treatment with elastase gave 2 amino acids and a tetrapeptide of the same composition werealso released.D. When the hexapeptide was also treated with cyanogen bromide, a dipeptide and atetrapeptide also of the same composition were also released.Questions:1. What is the sequence of the hexapeptide if it is composed of ser, met, ala and arg only?2. What is the pl of the hexapeptide?3. What is its charge at physiological pH (6.7-7.4) and where would you find this in a laneof an electropherogram?

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Answered: A hexapeptide of unknown sequence was…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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The authors state: "Properties of the single mutants K557R, D591V and K617A, a double mutant, D591V/K617A, and a triple mutant, K557R/D591V/K617A, were evaluated to find an allosteric binding site for citrate." The numbers between the letters represent the residue number altered. The letter in front was the wild type and the letter afterwards is the mutant. How would the the triple mutations K557R and D591V and K617A alter the overall protein? O lower the # of charges, increase the pl O increase the # of charges, increase the pl O lower the # of charges, lower the pl O lower the # of charges, increase the pl O no change in the # of charges, increase the pl O no change in the # of charges, lower the pl
What can we most-accurately say about the polypeptide with the primary sequence KNEADING? a.It has two acidic R groups b.It has three basic R groups c. It has two nonpolar neutral R groups d. It contains three polar neutral R groups e. It contains a disulfide linkage
In large-genome sequencing projects, the initial data usually reveal gaps where no sequence information has been obtained. To close the gaps, DNAprimers complementary to the 5′-ending strand (that is, identical to the sequence of the 3′-ending strand) at the end of each contig are especially useful. Explain how these primers might be used.
A bloactlve hexapeptide was cleaved by trypsln from a larger proteln. The peptlde was sequenced by treatment with varlOus reagents and enzymes. The results are as follows: a) Treatment with cyan0gen bromlde gave a tetrapeptlde of compositlon asp, trp, cys and met. b) Treatment with chymotrypsln gave a tripeptlde of compositlon asp, trp and cys, a dipeptlde wlth the same C-term and an amlno acld. c) Treatment with streptococcal protease gave a dipeptlde and a tetrapeptlde. The composltion of the peptlde Is lys, trp, asp, cys and met 1. What Is the sequence of the hexapeptlde? 2. What is the pl of thls peptlde?
Based on this alignment, you can identify areas of the protein sequences that are key regions of the proteins. If you were to design a targeted mutagenesis programme to try to disrupt the activity of the proteins in this current alignment as part of a research programme, which THREE amino acid sequence regions (motifs) would you target? List your sequence motifs in order of preference (number 1 being your most confident target). Explain your reasoning for each motif
Using the formulae for dsDNA to calculate g/mol: You have a 4110 bp cloning vector Want to ligate a 245 bp insert for molecular cloning. efficient cloning requires that a 1:5 molar ratio of vector to insert is optimal for the ligation reaction. What are the relative weights of vector and the insert DNA necessary in g?
There are three names that often get used when discussing the Lowry assay. Those names are: Lowry, Folin-Ciocalteu, and Biuret. Are those names interchangeable when discussing the assay or do those names describe the same thing(s)? Significant differences? What is the relationship between those names?
Describe Shine Dalgarno sequences?
From the BLAST results, identify the organism, gene, chromosome and accession number for this partial RefSeq.
The amino acid sequences of peptide fragments obtained from a normal protein were compared with those obtained from the same protein synthesized by a defective gene. They were found to differ in only one peptide fragment. Their amino acid sequences are shown here: Normal: Gln-Tyr-Gly-Thr-Arg-Tyr-Val Mutant: Gln-Ser-Glu-Pro-Gly-Thr a. What is the defect in DNA?b. It was later determined that the normal peptide fragment is an octapeptide with a C-terminal Val-Leu. What is the C-terminal amino acid of the mutant peptide?

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