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A hexapeptide of unknown sequence was isolated from an amphibian skin then sequenced. The following were the results of the sequencing process: A. When the peptide was treated with dinitrofluorobenzene (DNFB), DNP-ala and a mixture of amino acids were produced. B. When the same intact peptide was treated with trypsin, a tetrapeptide of composition arg, met, ala and ser and a dipeptide were released. C. Treatment with elastase gave 2 amino acids and a tetrapeptide of the same composition were also released. D. When the hexapeptide was also treated with cyanogen bromide, a dipeptide and a tetrapeptide also of the same composition were also released. Questions: 1. What is the sequence of the hexapeptide if it is composed of ser, met, ala and arg only? 2. What is the pl of the hexapeptide? 3. What is its charge at physiological pH (6.7-7.4) and where would you find this in a lane of an electropherogram?

A hexapeptide of unknown sequence was isolated from an amphibian skin then sequenced. The following were the results of the sequencing process: A. When the peptide was treated with dinitrofluorobenzene (DNFB), DNP-ala and a mixture of amino acids were produced. B. When the same intact peptide was treated with trypsin, a tetrapeptide of composition arg, met, ala and ser and a dipeptide were released. C. Treatment with elastase gave 2 amino acids and a tetrapeptide of the same composition were also released. D. When the hexapeptide was also treated with cyanogen bromide, a dipeptide and a tetrapeptide also of the same composition were also released. Questions: 1. What is the sequence of the hexapeptide if it is composed of ser, met, ala and arg only? 2. What is the pl of the hexapeptide? 3. What is its charge at physiological pH (6.7-7.4) and where would you find this in a lane of an electropherogram?

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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A hexapeptide of unknown sequence was isolated from an amphibian skin then sequenced. The following were the results of the sequencing process: A. When the peptide was treated with dinitrofluorobenzene (DNFB), DNP-ala and a mixture of amino acids were produced. B. When the same intact peptide was treated with trypsin, a tetrapeptide of composition arg, met, ala and ser and a dipeptide were released. C. Treatment with elastase gave 2 amino acids and a tetrapeptide of the same composition were also released. D. When the hexapeptide was also treated with cyanogen bromide, a dipeptide and a tetrapeptide also of the same composition were also released. Questions: 1. What is the sequence of the hexapeptide if it is composed of ser, met, ala and arg only? 2. What is the pl of the hexapeptide? 3. What is its charge at physiological pH (6.7-7.4) and where would you find this in a lane of an electropherogram?
Transcribed Image Text:A hexapeptide of unknown sequence was isolated from an amphibian skin then sequenced. The following were the results of the sequencing process: A. When the peptide was treated with dinitrofluorobenzene (DNFB), DNP-ala and a mixture of amino acids were produced. B. When the same intact peptide was treated with trypsin, a tetrapeptide of composition arg, met, ala and ser and a dipeptide were released. C. Treatment with elastase gave 2 amino acids and a tetrapeptide of the same composition were also released. D. When the hexapeptide was also treated with cyanogen bromide, a dipeptide and a tetrapeptide also of the same composition were also released. Questions: 1. What is the sequence of the hexapeptide if it is composed of ser, met, ala and arg only? 2. What is the pl of the hexapeptide? 3. What is its charge at physiological pH (6.7-7.4) and where would you find this in a lane of an electropherogram?
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