Apeptide was sequenced and the sequence of fragment sets generated by different cleavage methods is shown below. What is the primary structures of the peptide? Method 1: GAQIL + IALNGAS + TIRAGAGGMG + DIGCFEKAI Method 2: FEKAIIALNG + DIGC + GMGGAQIL + ASTIRAGAG Method 3: GGMGG + IALNGASTIRAGA + DIGCF + AQIL + EKAI O AGAGGAIIALNGASTIRMGGAQILDIGCFEK O AIIALNGASTIRMGGAQILDIGCFEKAGAGG O MGGAQILDKAIIALNGASTIRAGAGGIGCFE O DIGCFEKAIIALNGASTIRAGAGGMGGAQIL

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
icon
Related questions
Question
**Peptide Sequencing Challenge**

A peptide was sequenced, and the sequence of fragment sets generated by different cleavage methods is shown below. What is the primary structure of the peptide?

**Cleavage Methods and Fragment Sets:**

- **Method 1:**  
  - Fragments: GAQIL + IALNGAS + TIRAGAGGMG + DIGCFEKAI

- **Method 2:**  
  - Fragments: FEKAIIALNG + DIGC + GMGGAQIL + ASTIRAGAG

- **Method 3:**  
  - Fragments: GGMGG + IALNGASTIRAGA + DIGCF + AQIL + EKAI

**Options for Primary Structure of the Peptide:**

- ○ AGAGGAIIALNGASTIRMGGAQILDIGCFEK
- ○ AIIALNGASTIRMGGAQILDIGCFEKAGAGG
- ○ MGGAQILDKAIIALNGASTIRAGAGGIGCFE
- ○ DIGCFEKAIIALNGASTIRAGAGGMGGAQIL

Explore these fragments and determine the primary sequence of the peptide based on the given methods.
Transcribed Image Text:**Peptide Sequencing Challenge** A peptide was sequenced, and the sequence of fragment sets generated by different cleavage methods is shown below. What is the primary structure of the peptide? **Cleavage Methods and Fragment Sets:** - **Method 1:** - Fragments: GAQIL + IALNGAS + TIRAGAGGMG + DIGCFEKAI - **Method 2:** - Fragments: FEKAIIALNG + DIGC + GMGGAQIL + ASTIRAGAG - **Method 3:** - Fragments: GGMGG + IALNGASTIRAGA + DIGCF + AQIL + EKAI **Options for Primary Structure of the Peptide:** - ○ AGAGGAIIALNGASTIRMGGAQILDIGCFEK - ○ AIIALNGASTIRMGGAQILDIGCFEKAGAGG - ○ MGGAQILDKAIIALNGASTIRAGAGGIGCFE - ○ DIGCFEKAIIALNGASTIRAGAGGMGGAQIL Explore these fragments and determine the primary sequence of the peptide based on the given methods.
Expert Solution
Step 1

Amino acids are the molecules that join to form a protein structure. The bond formed between two amino acids is termed as peptide bond. There are total 20 amino acids, due to which probability of formation of number of proteins from combination of these amino acids is large.

steps

Step by step

Solved in 2 steps with 1 images

Blurred answer
Similar questions
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON