Under most in vitro assay conditions, the enzyme is used in catalytic amounts (10 to 10" M). Estimate the concentration of an enzyme in a living cell. Assume that (a) fresh tissue is 80% water and all of it is intracelular, (b) the total soluble protein in a cell represents 15% of the wet weight, (c) all the soluble proteins are enzymes, (d) the average molecular weight of a protein is 150,000, and (e) about 1000 different enzymes are present.
Proteins
We generally tend to think of proteins only from a dietary lens, as a component of what we eat. However, they are among the most important and abundant organic macromolecules in the human body, with diverse structures and functions. Every cell contains thousands and thousands of proteins, each with specific functions. Some help in the formation of cellular membrane or walls, some help the cell to move, others act as messages or signals and flow seamlessly from one cell to another, carrying information.
Protein Expression
The method by which living organisms synthesize proteins and further modify and regulate them is called protein expression. Protein expression plays a significant role in several types of research and is highly utilized in molecular biology, biochemistry, and protein research laboratories.
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