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A laboratory in Japan performed a similar experiment on earthworms from different localities of the Japanese archipelago, but instead of linking trypsin activity within worms to anatomical features, they were interested in geographical differences in the ability of the worms to breakdown environmental pollutants using the enzyme polyphenol oxidase. The protocol was similar to the one you used, except that enzyme activity was measured from 100 µl of worm extract. Data obtained are displayed below: Replicate | Amount of product liberated over 10 minutes (pmol) Concentration of total protein in worm extract (µg/ml) Locality |Hokkaido 1 101 4 87 107 3 3 4 4 88 3 5 89 5 Hon-shu 126 120 1 3 3 156 4 153 119 4 5 |5 4 Kyu-shu 1 103 4 99 5 3 94 4 108 3 5 110 | Okinawa 88 3 2 3 90 4 96 4 5 100 112 3 From the data in the table, calculate the specific activity of polyphenol oxidase (in units of umol/min/mg protein) from each locality, presenting the data as mean ± standard error (n=5).

A laboratory in Japan performed a similar experiment on earthworms from different localities of the Japanese archipelago, but instead of linking trypsin activity within worms to anatomical features, they were interested in geographical differences in the ability of the worms to breakdown environmental pollutants using the enzyme polyphenol oxidase. The protocol was similar to the one you used, except that enzyme activity was measured from 100 µl of worm extract. Data obtained are displayed below: Replicate | Amount of product liberated over 10 minutes (pmol) Concentration of total protein in worm extract (µg/ml) Locality |Hokkaido 1 101 4 87 107 3 3 4 4 88 3 5 89 5 Hon-shu 126 120 1 3 3 156 4 153 119 4 5 |5 4 Kyu-shu 1 103 4 99 5 3 94 4 108 3 5 110 | Okinawa 88 3 2 3 90 4 96 4 5 100 112 3 From the data in the table, calculate the specific activity of polyphenol oxidase (in units of umol/min/mg protein) from each locality, presenting the data as mean ± standard error (n=5).

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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A laboratory in Japan performed a similar experiment on earthworms from different localities of the Japanese archipelago, but instead of linking trypsin activity within worms to anatomical features, they were interested in geographical differences in the ability of the worms to breakdown environmental pollutants using the enzyme polyphenol oxidase. The protocol was similar to the one you used, except that enzyme activity was measured from 100 µl of worm extract. Data obtained are displayed below: Locality Replicate Amount of product Concentration of total protein in worm extract (µg/ml) liberated over 10 minutes (pmol) Hokkaido 1 101 4 87 3 3 107 4 4 88 3 5 89 Hon-shu 1 126 2 2 120 3 3 156 4 4 153 5 5 119 4 Куu-shu 1 103 4 2 99 5 3 94 4 108 3 5 110 5 Okinawa 1 88 3 2 90 4 3 96 4 100 112 5 5 3 From the data in the table, calculate the specific activity of polyphenol oxidase (in units of umol/min/mg protein) from each locality, presenting the data as mean + standard error (n=5).
Transcribed Image Text:A laboratory in Japan performed a similar experiment on earthworms from different localities of the Japanese archipelago, but instead of linking trypsin activity within worms to anatomical features, they were interested in geographical differences in the ability of the worms to breakdown environmental pollutants using the enzyme polyphenol oxidase. The protocol was similar to the one you used, except that enzyme activity was measured from 100 µl of worm extract. Data obtained are displayed below: Locality Replicate Amount of product Concentration of total protein in worm extract (µg/ml) liberated over 10 minutes (pmol) Hokkaido 1 101 4 87 3 3 107 4 4 88 3 5 89 Hon-shu 1 126 2 2 120 3 3 156 4 4 153 5 5 119 4 Куu-shu 1 103 4 2 99 5 3 94 4 108 3 5 110 5 Okinawa 1 88 3 2 90 4 3 96 4 100 112 5 5 3 From the data in the table, calculate the specific activity of polyphenol oxidase (in units of umol/min/mg protein) from each locality, presenting the data as mean + standard error (n=5).
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