answer the following questioms.. 1.What is the action of invertase? 2.What is the reagent used to detect glucose in the detection of invertase? What is its principle?
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answer the following questioms..
1.What is the action of invertase?
2.What is the reagent used to detect glucose in the detection of invertase? What is its principle?
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- One of your colleagues has obtained a sample of muscle phosphorylase b that is known to be relatively inactive. She has approached you for advice on how to set up an appropriate assay. She has the following items available, not all of which are appropriate for this study. Help her out by selecting the items that she should use and what their purpose is in the assay, and then explain why each of the other items would not be useful. 1. 100 UM AMP 2. 100 UM GTP 3.100 uM glucose 4. 100 uM glucose 6-phosphate 5. Branched glycogen 6. Amylose (i.e. unbranched glycogen) 7.50 mM HEPES buffer, pH 7.5 8. 50 mM potassium phosphate buffer, pH 7.5 Write out a short explanation for each of the above items, and upload your answers by the due date.Serum blood of a patient with dislipoproteinemia type 1 has milky appearance even in fasting. If serum stays at low temperature (40) for several hours fatty layer appears on its surface. What are the possible causes of these symptoms? To explain this, answer the questions and do the following tasks: a) what compounds of serum must be tested for that patient in biochemical lab? b) write the reaction which does not occur properly in patient’s blood; c) write down the schemes, explaining how the products of the previous reaction are used in adipose tissue and heart in healthy person 2 hours after a meal.Ibuprofen and indomethacin are clinically important inhibitors of prostaglandin H₂ synthase-1. Cells expressing this enzyme were incubated under four different conditions, after which the activity of the enzyme was measured by adding radiolabeled arachidonic acid and detecting newly-produced prostaglandin H₂: 1. 40 min without inhibitor (control) 2. 40 min with inhibitor 3. 40 min with inhibitor, after which the cells were resuspended in medium without inhibitor 4. 40 min with inhibitor, after which the cells were resuspended in medium without inhibitor and incubated for an additional 30 min The bar graphs show the results of the experiments with ibuprofen and indomethacin. The bars in each graph correspond to the four different incubation conditions. Ibuprofen Prostaglandin H₂ synthesized (relative to control) 100 80 60 40 20 I I I (1) (2) ll (3) (4) Prostaglandin H₂ synthesized (relative to control) 100k 80 60 40 20 I (1) Indomethacin (2) (3) (4)
- One of the blocking solutions that is traditionally used is non-fat milk, but this is not recommended for studies of phosphoproteins. Why?A protein (PrX) was heated in vitro to 45C for 15 min, in the presence or absence of Hsp40, Hsc70, and ATP. The amount of PrX in aggregated (P, pellet) and soluble (S) fractions was then quantified (Bars, 1 SD). Based on these data, answer the following questions. In all cases, give brief justification for your answer. a) How does Hsp40 alone influence the solubility of PrX? b) How does Hsc70 alone influence the solubility of PrX? c) Do Hsp40 and Hsc70 co-operate in this experiment? d) Which components combined provide maximal solubility of PrX after heat shock?1. What are the primary tissue sources of amylase? 2. What is the clinical significance of increase serum amylase activity? 3. What are the assay methodologies? Describe each.
- Tyrosinase enzyme activity is assayed by monitoring the oxidation of 3, 4-dihydroxyphenylalanine (dopa) to the red-colored dopachrome. Calculate the tyrosinase activity (U/mL) by using the experimental data given belowThe substrate which was used to investigate the enzymatic activity of wheat bran phosphatase was p-nitrophenyl phosphate. It is converted to p-nitrophenol and inorganic phosphate by this enzyme. P-nitrophenol has a pKa of 7.1 and is colorless, while its conjugate base, p-nitrophenolate has a yellow color. The enzyme assay was performed at pH 5.1 after the 0.5 N KOH is added at the end of each enzyme assay, the pH of the assay mixture is raised to 9.1. Why was the base added?1. What is the action of invertase? 2. What is the reagent used to detect glucose in the detection of invertase? What is its principle? 3. What is the reagent used to detect glucose in the oh and temperature experiments? What is its principle? 4. What is the optimum temperature for invertase reactions? 5. What is the optimum pH for the invertase reactions? 6. What is the wavelength used for the spectrophotometer? What is the principle of the spectrophotometer?
- Catalase is the enzyme. experiment with h2O2 3. What is the marker for the enzyme’s optimum condition?There are several applications of microorganisms in industries. One of them is in medical and pharmaceuticals. Name a suitable microorganism and demonstrate its application in producing synthetic human insulin.1. What is the action of invertase? 2. What is the reagent used to detect glucose in the detection of invertase? What is its principle?