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Measure the uptake of leucine by epithetial cells of the mouse intestine. rements of the rate of update of L-leucine, D-Leucine, and L-valine , with and without the assay were perform and yield different results (see table below). A) What can you de about the properties and mechanism of leucine transporter? B) Would you expect ine uptake to be inhibited by Quabain, which is a cardiac glycoside drug treatment?

Measure the uptake of leucine by epithetial cells of the mouse intestine. rements of the rate of update of L-leucine, D-Leucine, and L-valine , with and without the assay were perform and yield different results (see table below). A) What can you de about the properties and mechanism of leucine transporter? B) Would you expect ine uptake to be inhibited by Quabain, which is a cardiac glycoside drug treatment?

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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**Title: Leucine Uptake by Epithelial Cells in Mouse Intestine: Experimental Insights** **Introduction:** This study focuses on measuring the uptake of leucine by epithelial cells in the mouse intestine. The experiment examines the rate of uptake of L-leucine, D-leucine, and L-valine, both with and without the presence of sodium ions (Na+) in the assay. Different results were obtained, providing valuable insights into the properties and mechanisms of the leucine transporter. **Key Questions and Analysis:** **A) Properties and Mechanism of Leucine Transporter:** - The experiment provides information on how the presence or absence of Na+ affects the uptake rates of different amino acids. - Insights can be drawn on whether the leucine transporter requires Na+ for optimal functionality and if it shows any stereospecificity for L- versus D-enantiomers of leucine. **B) Inhibition by Ouabain:** - Ouabain is a cardiac glycoside known for its impact on Na+/K+ ATPase. - The experiment evaluates whether L-leucine uptake is inhibited by ouabain, suggesting a potential dependence on Na+/K+ gradients maintained by the enzyme. **Conclusion:** The findings are crucial for understanding the transport mechanisms of essential amino acids in intestinal cells, potentially influencing nutritional and pharmacological strategies. For a detailed examination of the results, please refer to the supplementary table below. (Note: Since there was no visible table provided in this exercise, ensure to include any relevant data tables when presenting on an educational platform.)
Transcribed Image Text:**Title: Leucine Uptake by Epithelial Cells in Mouse Intestine: Experimental Insights** **Introduction:** This study focuses on measuring the uptake of leucine by epithelial cells in the mouse intestine. The experiment examines the rate of uptake of L-leucine, D-leucine, and L-valine, both with and without the presence of sodium ions (Na+) in the assay. Different results were obtained, providing valuable insights into the properties and mechanisms of the leucine transporter. **Key Questions and Analysis:** **A) Properties and Mechanism of Leucine Transporter:** - The experiment provides information on how the presence or absence of Na+ affects the uptake rates of different amino acids. - Insights can be drawn on whether the leucine transporter requires Na+ for optimal functionality and if it shows any stereospecificity for L- versus D-enantiomers of leucine. **B) Inhibition by Ouabain:** - Ouabain is a cardiac glycoside known for its impact on Na+/K+ ATPase. - The experiment evaluates whether L-leucine uptake is inhibited by ouabain, suggesting a potential dependence on Na+/K+ gradients maintained by the enzyme. **Conclusion:** The findings are crucial for understanding the transport mechanisms of essential amino acids in intestinal cells, potentially influencing nutritional and pharmacological strategies. For a detailed examination of the results, please refer to the supplementary table below. (Note: Since there was no visible table provided in this exercise, ensure to include any relevant data tables when presenting on an educational platform.)
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