In-class exercise: protein trafficking though the endomembrane systemNameYou are studying potential functions of the three proteins shown at the top of the display screens. You have acell line in which are able to turn transcription (DNA➜mRNA) on and off, then follow the fate of the geneproducts. You turn on transcription of all three genes for 30 minutes, then stop it. On average, it takes one hourfrom the beginning of transcription for the resulting protein to be fully processed in the Golgi apparatus. On thegraphs below, show the relative levels of red, blue and green fluorescence you expect to see in the six cellularlocations indicated. Show the expectations at the point you turn off transcription in the top graph, one hour laterin the middle graph and five hours after on the bottom.Time transcription is stoppedPercent of total fluorescence100Percent of total fluorescence50Percent of total fluorescenceO1001850100Nucleus50NucleusNucleusRough ERRough ERSmooth EROne hour after end of transcriptionRough ERSmooth ERGolgiFive hours after end of transcriptionSmooth ERGolgiGolgiLysosomeLysosomeLysosomePlasmamembranePlasmamembranePlasmamembrane Three proteins synthesized byER-associated ribosomes(translation)Receptors in trans-face ofthe Golgi apparatusReceptor inplasmamembraneReceptor inlysosomemembraneReceptor insmooth ER

Name: In-class exercise: protein trafficking though the endomembrane system
Uploaded: 2024-03-20T06:42:41.000Z
Channel: Bartleby
Description: In-class exercise: protein trafficking though the endomembrane systemNameYou are studying potential functions of the three proteins shown at the top of the display screens. You have acell line in which are able to turn transcription (DNA➜mRNA) on an