Below is the double-stranded DNA sequence of part of a hypothetical yeast genome, which happens to contain a very small gene. Transcription starts at the Transcription Start Site (TSS) after the promoter (in yellow) and proceeds in the direction of the arrow. Transcription stops at the end of the Transcription Terminator sequence on the right (in blue). 1. TSS 5 -CTATAAAGAGCCATGCATTATCTAGATAGTAGGCTCTGAGAATTTATCTCACT-3' TI||||| 3'-GATATTTCTCGGTACGTAATAGATCTATCATCCGAGACTCTTAAÄTAGAGTGA-5' Which strand of DNA shown, the top or the bottom, is the template strand? Explain 'briefly why a) b) Which strand of DNA shown, the top or the bottom, is the coding strand? c) Write the first 10 bases of the sequence of the MRNA produced from this gene? Label the 5' and 3' ends?

Human Anatomy & Physiology (11th Edition)
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Chapter1: The Human Body: An Orientation
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Below is the double-stranded DNA sequence of part of a hypothetical yeast genome, which
happens to contain a very small gene. Transcription starts at the Transcription Start Site (TSS)
after the promoter (in yellow) and proceeds in the direction of the arrow. Transcription stops at
the end of the Transcription Terminator sequence on the right (in blue).
1.
TSS
5' -СТАТАAGAGCCATGCATTATCTAGATAGTAGGCTCTGAGAAТТТАТСТСАСТ-3'
3'-GATATTTCTCGGTACGTAATAGATCTATCATCCGAGACTCTTAAATAGAGTGA-5'
Which strand of DNA shown, the top or the bottom, is the template strand? Explain
*briefly why
a)
b)
Which strand of DNA shown, the top or the bottom, is the coding strand?
c)
Write the first 10 bases of the sequence of the mRNA produced from this gene? Label
the 5' and 3' ends?
If a mutation were found where additional five G/C (top/bottom) base pairs were
inserted immediately after the T/A base pair shown in bold in the DNA, what would be
d)
the effect on the size of the mutated mRNA?
If a mutation were found where 5 base pairs were deleted immediately after the T/A
base pair shown in bold, in the DNA what would be the effect on the size of the mutated
MRNA?
If a mutation were found where 5 base pairs were deleted upstream or before the
transcriptional start site, in the promoter region. What would be the effect on gene
expression? Explain briefly.
f)
Transcribed Image Text:Below is the double-stranded DNA sequence of part of a hypothetical yeast genome, which happens to contain a very small gene. Transcription starts at the Transcription Start Site (TSS) after the promoter (in yellow) and proceeds in the direction of the arrow. Transcription stops at the end of the Transcription Terminator sequence on the right (in blue). 1. TSS 5' -СТАТАAGAGCCATGCATTATCTAGATAGTAGGCTCTGAGAAТТТАТСТСАСТ-3' 3'-GATATTTCTCGGTACGTAATAGATCTATCATCCGAGACTCTTAAATAGAGTGA-5' Which strand of DNA shown, the top or the bottom, is the template strand? Explain *briefly why a) b) Which strand of DNA shown, the top or the bottom, is the coding strand? c) Write the first 10 bases of the sequence of the mRNA produced from this gene? Label the 5' and 3' ends? If a mutation were found where additional five G/C (top/bottom) base pairs were inserted immediately after the T/A base pair shown in bold in the DNA, what would be d) the effect on the size of the mutated mRNA? If a mutation were found where 5 base pairs were deleted immediately after the T/A base pair shown in bold, in the DNA what would be the effect on the size of the mutated MRNA? If a mutation were found where 5 base pairs were deleted upstream or before the transcriptional start site, in the promoter region. What would be the effect on gene expression? Explain briefly. f)
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