LOCUS HUMTFIID 1876 bp mRNA linear PRI 03-AUG-1993
DEFINITION Human TATA-binding protein mRNA, complete cds.
ACCESSION M55654
VERSION M55654.1
KEYWORDS TATA binding protein.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1876)
AUTHORS Peterson,M.G., Tanese,N., Pugh,B.F. and Tjian,R.
TITLE Functional domains and upstream activation properties of cloned
human TATA binding protein
JOURNAL Science 248 (4963), 1625-1630 (1990)
PUBMED 2363050
COMMENT Original source text: Human, cDNA to mRNA.
FEATURES Location/Qualifiers
source 1..1876
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
CDS 242..1261
/codon_start=1
/product="TATA-box binding protein"
/protein_id="AAA36731.1"
/translation="MDQNNSLPPYAQGLASPQGAMTPGIPIFSPMMPYGTGLTPQPIQ
NTNSLSILEEQQRQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQAVAAAAV
QQSTSQQATQGTSGQAPQLFHSQTLTTAPLPGTTPLYPSPMTPMTPITPATPASESSG
IVPQLQNIVSTVNLGCKLDLKTIALRARNAEYNPKRFAAVIMRIREPRTTALIFSSGK
MVCTGAKSEEQSRLAARKYARVVQKLGFPAKFLDFKIQNMVGSCDVKFPIRLEGLVLT
HQQFSSYEPELFPGLIYRMIKPRIVLLIFVSGKVVLTGAKVRAEIYEAFENIYPILKG
FRKTT"
ORIGIN
1 cgcggccgcg gttcgctgtg gcgggcgcct gggccgccgg ctgtttaact tcgcttccgc
61 tggcccatag tgatctttgc agtgacccag cagcatcact gtttcttggc gtgtgaagat
121 aacccaagga attgaggaag ttgctgagaa gagtgtgctg gagatgctct aggaaaaaat
181 tgaatagtga gacgagttcc agcgcaaggg tttctggttt gccaagaaga aagtgaacat
241 catggatcag aacaacagcc tgccacctta cgctcagggc ttggcctccc ctcagggtgc
301 catgactccc ggaatcccta tctttagtcc aatgatgcct tatggcactg gactgacccc
361 acagcctatt cagaacacca atagtctgtc tattttggaa gagcaacaaa ggcagcagca
421 gcaacaacaa cagcagcagc agcagcagca gcagcagcaa cagcaacagc agcagcagca
481 gcagcagcag cagcagcagc agcagcagca gcagcagcag caacaggcag tggcagctgc
541 agccgttcag cagtcaacgt cccagcaggc aacacaggga acctcaggcc aggcaccaca
601 gctcttccac tcacagactc tcacaactgc acccttgccg ggcaccactc cactgtatcc
661 ctcccccatg actcccatga cccccatcac tcctgccacg ccagcttcgg agagttctgg
721 gattgtaccg cagctgcaaa atattgtatc cacagtgaat cttggttgta aacttgacct
781 aaagaccatt gcacttcgtg cccgaaacgc cgaatataat cccaagcggt ttgctgcggt
841 aatcatgagg ataagagagc cacgaaccac ggcactgatt ttcagttctg ggaaaatggt
901 gtgcacagga gccaagagtg aagaacagtc cagactggca gcaagaaaat atgctagagt
961 tgtacagaag ttgggttttc cagctaagtt cttggacttc aagattcaga acatggtggg
1021 gagctgtgat gtgaagtttc ctataaggtt agaaggcctt gtgctcaccc accaacaatt
1081 tagtagttat gagccagagt tatttcctgg tttaatctac agaatgatca aacccagaat
1141 tgttctcctt atttttgttt ctggaaaagt tgtattaaca ggtgctaaag tcagagcaga
1201 aatttatgaa gcatttgaaa acatctaccc tattctaaag ggattcagga agacgacgta
1261 atggctctca tgtacccttg cctcccccac ccccttcttt tttttttttt aaacaaatca
1321 gtttgttttg gtacctttaa atggtggtgt tgtgagaaga tggatgttga gttgcagggt
1381 gtggcaccag gtgatgccct tctgtaagtg cccaccgcgg gatgccggga aggggcatta
1441 tttgtgcact gagaacaccg cgcagcgtga ctgtgagttg ctcataccgt gctgctatct
1501 gggcagcgct gcccatttat ttatatgtag attttaaaca ctgctgttga caagttggtt
1561 tgagggagaa aactttaagt gttaaagcca cctctataat tgattggact ttttaatttt
1621 aatgtttttc cccatgaacc acagttttta tatttctacc agaaaagtaa aaatcttttt
1681 taaaagtgtt gtttttctaa tttataactc ctaggggtta tttctgtgcc agacacattc
1741 cacctctcca gtattgcagg acggaatata tgtgttaatg aaaatgaatg gctgtacata
1801 tttttttctt tcttcagagt actctgtaca ataaatgcag tttataaaag tgttaaaaaa
1861 aaaaaaaaaa aaaaaa
Above is the sequence for TBP.
Abbreviations CDS-coding sequence
- What is the biological function of this gene?
- How long is the coding sequence of this gene?
- What is the position of the
nucleotides that encode the start codon - Where is the position of the nucleotides that encode stop codon?
- How many amino acids make up this protein?
- Design an upstream and downstream primer to amplify nucleotides 242-1261.
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