LOCUS HUMTFIID 1876 bp mRNA linear PRI 03-AUG-1993 DEFINITION Human TATA-binding protein mRNA, complete cds. ACCESSION M55654 VERSION M55654.1 KEYWORDS TATA binding protein. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 1876) AUTHORS Peterson,M.G., Tanese,N., Pugh,B.F. and Tjian,R. TITLE Functional domains and upstream activation properties of cloned human TATA binding protein JOURNAL Science 248 (4963), 1625-1630 (1990) PUBMED 2363050 COMMENT Original source text: Human, cDNA to mRNA. FEATURES Location/Qualifiers source 1..1876 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" CDS 242..1261 /codon_start=1 /product="TATA-box binding protein" /protein_id="AAA36731.1" /translation="MDQNNSLPPYAQGLASPQGAMTPGIPIFSPMMPYGTGLTPQPIQ NTNSLSILEEQQRQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQAVAAAAV QQSTSQQATQGTSGQAPQLFHSQTLTTAPLPGTTPLYPSPMTPMTPITPATPASESSG IVPQLQNIVSTVNLGCKLDLKTIALRARNAEYNPKRFAAVIMRIREPRTTALIFSSGK MVCTGAKSEEQSRLAARKYARVVQKLGFPAKFLDFKIQNMVGSCDVKFPIRLEGLVLT HQQFSSYEPELFPGLIYRMIKPRIVLLIFVSGKVVLTGAKVRAEIYEAFENIYPILKG FRKTT" ORIGIN 1 cgcggccgcg gttcgctgtg gcgggcgcct gggccgccgg ctgtttaact tcgcttccgc 61 tggcccatag tgatctttgc agtgacccag cagcatcact gtttcttggc gtgtgaagat 121 aacccaagga attgaggaag ttgctgagaa gagtgtgctg gagatgctct aggaaaaaat 181 tgaatagtga gacgagttcc agcgcaaggg tttctggttt gccaagaaga aagtgaacat 241 catggatcag aacaacagcc tgccacctta cgctcagggc ttggcctccc ctcagggtgc 301 catgactccc ggaatcccta tctttagtcc aatgatgcct tatggcactg gactgacccc 361 acagcctatt cagaacacca atagtctgtc tattttggaa gagcaacaaa ggcagcagca 421 gcaacaacaa cagcagcagc agcagcagca gcagcagcaa cagcaacagc agcagcagca 481 gcagcagcag cagcagcagc agcagcagca gcagcagcag caacaggcag tggcagctgc 541 agccgttcag cagtcaacgt cccagcaggc aacacaggga acctcaggcc aggcaccaca 601 gctcttccac tcacagactc tcacaactgc acccttgccg ggcaccactc cactgtatcc 661 ctcccccatg actcccatga cccccatcac tcctgccacg ccagcttcgg agagttctgg 721 gattgtaccg cagctgcaaa atattgtatc cacagtgaat cttggttgta aacttgacct 781 aaagaccatt gcacttcgtg cccgaaacgc cgaatataat cccaagcggt ttgctgcggt 841 aatcatgagg ataagagagc cacgaaccac ggcactgatt ttcagttctg ggaaaatggt 901 gtgcacagga gccaagagtg aagaacagtc cagactggca gcaagaaaat atgctagagt 961 tgtacagaag ttgggttttc cagctaagtt cttggacttc aagattcaga acatggtggg 1021 gagctgtgat gtgaagtttc ctataaggtt agaaggcctt gtgctcaccc accaacaatt 1081 tagtagttat gagccagagt tatttcctgg tttaatctac agaatgatca aacccagaat 1141 tgttctcctt atttttgttt ctggaaaagt tgtattaaca ggtgctaaag tcagagcaga 1201 aatttatgaa gcatttgaaa acatctaccc tattctaaag ggattcagga agacgacgta 1261 atggctctca tgtacccttg cctcccccac ccccttcttt tttttttttt aaacaaatca 1321 gtttgttttg gtacctttaa atggtggtgt tgtgagaaga tggatgttga gttgcagggt 1381 gtggcaccag gtgatgccct tctgtaagtg cccaccgcgg gatgccggga aggggcatta 1441 tttgtgcact gagaacaccg cgcagcgtga ctgtgagttg ctcataccgt gctgctatct 1501 gggcagcgct gcccatttat ttatatgtag attttaaaca ctgctgttga caagttggtt 1561 tgagggagaa aactttaagt gttaaagcca cctctataat tgattggact ttttaatttt 1621 aatgtttttc cccatgaacc acagttttta tatttctacc agaaaagtaa aaatcttttt 1681 taaaagtgtt gtttttctaa tttataactc ctaggggtta tttctgtgcc agacacattc 1741 cacctctcca gtattgcagg acggaatata tgtgttaatg aaaatgaatg gctgtacata 1801 tttttttctt tcttcagagt actctgtaca ataaatgcag tttataaaag tgttaaaaaa 1861 aaaaaaaaaa aaaaa Design an upstream and downstream primer to amplify nucleotides 242-1261.
LOCUS HUMTFIID 1876 bp mRNA linear PRI 03-AUG-1993
DEFINITION Human TATA-binding protein mRNA, complete cds.
ACCESSION M55654
VERSION M55654.1
KEYWORDS TATA binding protein.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1876)
AUTHORS Peterson,M.G., Tanese,N., Pugh,B.F. and Tjian,R.
TITLE Functional domains and upstream activation properties of cloned
human TATA binding protein
JOURNAL Science 248 (4963), 1625-1630 (1990)
PUBMED 2363050
COMMENT Original source text: Human, cDNA to mRNA.
FEATURES Location/Qualifiers
source 1..1876
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
CDS 242..1261
/codon_start=1
/product="TATA-box binding protein"
/protein_id="AAA36731.1"
/translation="MDQNNSLPPYAQGLASPQGAMTPGIPIFSPMMPYGTGLTPQPIQ
NTNSLSILEEQQRQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQAVAAAAV
QQSTSQQATQGTSGQAPQLFHSQTLTTAPLPGTTPLYPSPMTPMTPITPATPASESSG
IVPQLQNIVSTVNLGCKLDLKTIALRARNAEYNPKRFAAVIMRIREPRTTALIFSSGK
MVCTGAKSEEQSRLAARKYARVVQKLGFPAKFLDFKIQNMVGSCDVKFPIRLEGLVLT
HQQFSSYEPELFPGLIYRMIKPRIVLLIFVSGKVVLTGAKVRAEIYEAFENIYPILKG
FRKTT"
ORIGIN
1 cgcggccgcg gttcgctgtg gcgggcgcct gggccgccgg ctgtttaact tcgcttccgc
61 tggcccatag tgatctttgc agtgacccag cagcatcact gtttcttggc gtgtgaagat
121 aacccaagga attgaggaag ttgctgagaa gagtgtgctg gagatgctct aggaaaaaat
181 tgaatagtga gacgagttcc agcgcaaggg tttctggttt gccaagaaga aagtgaacat
241 catggatcag aacaacagcc tgccacctta cgctcagggc ttggcctccc ctcagggtgc
301 catgactccc ggaatcccta tctttagtcc aatgatgcct tatggcactg gactgacccc
361 acagcctatt cagaacacca atagtctgtc tattttggaa gagcaacaaa ggcagcagca
421 gcaacaacaa cagcagcagc agcagcagca gcagcagcaa cagcaacagc agcagcagca
481 gcagcagcag cagcagcagc agcagcagca gcagcagcag caacaggcag tggcagctgc
541 agccgttcag cagtcaacgt cccagcaggc aacacaggga acctcaggcc aggcaccaca
601 gctcttccac tcacagactc tcacaactgc acccttgccg ggcaccactc cactgtatcc
661 ctcccccatg actcccatga cccccatcac tcctgccacg ccagcttcgg agagttctgg
721 gattgtaccg cagctgcaaa atattgtatc cacagtgaat cttggttgta aacttgacct
781 aaagaccatt gcacttcgtg cccgaaacgc cgaatataat cccaagcggt ttgctgcggt
841 aatcatgagg ataagagagc cacgaaccac ggcactgatt ttcagttctg ggaaaatggt
901 gtgcacagga gccaagagtg aagaacagtc cagactggca gcaagaaaat atgctagagt
961 tgtacagaag ttgggttttc cagctaagtt cttggacttc aagattcaga acatggtggg
1021 gagctgtgat gtgaagtttc ctataaggtt agaaggcctt gtgctcaccc accaacaatt
1081 tagtagttat gagccagagt tatttcctgg tttaatctac agaatgatca aacccagaat
1141 tgttctcctt atttttgttt ctggaaaagt tgtattaaca ggtgctaaag tcagagcaga
1201 aatttatgaa gcatttgaaa acatctaccc tattctaaag ggattcagga agacgacgta
1261 atggctctca tgtacccttg cctcccccac ccccttcttt tttttttttt aaacaaatca
1321 gtttgttttg gtacctttaa atggtggtgt tgtgagaaga tggatgttga gttgcagggt
1381 gtggcaccag gtgatgccct tctgtaagtg cccaccgcgg gatgccggga aggggcatta
1441 tttgtgcact gagaacaccg cgcagcgtga ctgtgagttg ctcataccgt gctgctatct
1501 gggcagcgct gcccatttat ttatatgtag attttaaaca ctgctgttga caagttggtt
1561 tgagggagaa aactttaagt gttaaagcca cctctataat tgattggact ttttaatttt
1621 aatgtttttc cccatgaacc acagttttta tatttctacc agaaaagtaa aaatcttttt
1681 taaaagtgtt gtttttctaa tttataactc ctaggggtta tttctgtgcc agacacattc
1741 cacctctcca gtattgcagg acggaatata tgtgttaatg aaaatgaatg gctgtacata
1801 tttttttctt tcttcagagt actctgtaca ataaatgcag tttataaaag tgttaaaaaa
1861 aaaaaaaaaa aaaaa
Design an upstream and downstream primer to amplify
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