**Recitation 8 - Spring 2022 B** The Holechek lab has sequenced a bacterial gene (LacZ) coding for a protein (β-galactosidase) that breaks down lactose into glucose and galactose. The sequence for the non-coding strand is as follows below, with spaces between every 10 base pairs: 5’ - TATCTAGA CACAGACCA AGTACGTGGA GCGGTTGCGC CGCCTCGACC GGAATTCCGG CAACATAGA GCGGCCGCAG TGTGACGAAA TCCACCCGGC TCCCCGCGCG TTGGCCGATC CAGCACGTGC TGCTCCTGTT CCAGTTCGTT GCCGCTTATC AACGGGTGGT GGTTACGCGC ACTATCGACT ACCTAGGCTT GATGAGCGTG ATATTAGGTG CACGCACGTG CTGCGGTGGC CTGACGCGCC ACGTGCCGGA AAGTGTGGCT CCCCGTTATT CCGGACTGAC TAGGCAGAAA TTCAGACCAC ACACTCCAGA ACGCCAGCGT GCGCTGCCGA ACGGTGCATA CAGGTGTCGT GTTGCGCAGC GGTCCGTTAT ATGGTACATA GTCTCCATAC CCGCCTGTTG CCGTCTGACT TCTGCAAGAC GCGCTGCTGG CTTTCTGGTG GGTGCCGCCC GAGAACAGCA ATGCGAAAAC GAAGCTCTCC GGTGACGTTG CGGACACCTC GGCGTGCACC GAATCTGGTC ATCGCCCATT GTTCGGCTTC GTCACGGCAA TATGTCCAGC AGTGTTCGCG GTTCGCTGGT GGCGAGGCTT GTTGTCGCAA CTACGCGTTC GCAGCCACCA TGGTCGCGTA GAAAGCCCAG TGTTACCCTG TACGACCGAC TACAACCACT GGGTACACCA TCCGTCCACC GTTGACCACA GCCGTCACCA TTACGACCGG ACACGTTTCC CTACTGGG

Biochemistry
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ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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**Recitation 8 - Spring 2022 B**

The Holechek lab has sequenced a bacterial gene (LacZ) coding for a protein (β-galactosidase) that breaks down lactose into glucose and galactose. The sequence for the non-coding strand is as follows below, with spaces between every 10 base pairs:

5’ - TATCTAGA CACAGACCA AGTACGTGGA GCGGTTGCGC CGCCTCGACC GGAATTCCGG  
CAACATAGA GCGGCCGCAG TGTGACGAAA TCCACCCGGC TCCCCGCGCG TTGGCCGATC  
CAGCACGTGC TGCTCCTGTT CCAGTTCGTT GCCGCTTATC AACGGGTGGT GGTTACGCGC  
ACTATCGACT ACCTAGGCTT GATGAGCGTG ATATTAGGTG CACGCACGTG CTGCGGTGGC  
CTGACGCGCC ACGTGCCGGA AAGTGTGGCT CCCCGTTATT CCGGACTGAC TAGGCAGAAA  
TTCAGACCAC ACACTCCAGA ACGCCAGCGT GCGCTGCCGA ACGGTGCATA CAGGTGTCGT  
GTTGCGCAGC GGTCCGTTAT ATGGTACATA GTCTCCATAC CCGCCTGTTG CCGTCTGACT  
TCTGCAAGAC GCGCTGCTGG CTTTCTGGTG GGTGCCGCCC GAGAACAGCA ATGCGAAAAC  
GAAGCTCTCC GGTGACGTTG CGGACACCTC GGCGTGCACC GAATCTGGTC ATCGCCCATT  
GTTCGGCTTC GTCACGGCAA TATGTCCAGC AGTGTTCGCG GTTCGCTGGT GGCGAGGCTT  
GTTGTCGCAA CTACGCGTTC GCAGCCACCA TGGTCGCGTA GAAAGCCCAG TGTTACCCTG  
TACGACCGAC TACAACCACT GGGTACACCA TCCGTCCACC GTTGACCACA GCCGTCACCA  
TTACGACCGG ACACGTTTCC CTACTGGG
Transcribed Image Text:**Recitation 8 - Spring 2022 B** The Holechek lab has sequenced a bacterial gene (LacZ) coding for a protein (β-galactosidase) that breaks down lactose into glucose and galactose. The sequence for the non-coding strand is as follows below, with spaces between every 10 base pairs: 5’ - TATCTAGA CACAGACCA AGTACGTGGA GCGGTTGCGC CGCCTCGACC GGAATTCCGG CAACATAGA GCGGCCGCAG TGTGACGAAA TCCACCCGGC TCCCCGCGCG TTGGCCGATC CAGCACGTGC TGCTCCTGTT CCAGTTCGTT GCCGCTTATC AACGGGTGGT GGTTACGCGC ACTATCGACT ACCTAGGCTT GATGAGCGTG ATATTAGGTG CACGCACGTG CTGCGGTGGC CTGACGCGCC ACGTGCCGGA AAGTGTGGCT CCCCGTTATT CCGGACTGAC TAGGCAGAAA TTCAGACCAC ACACTCCAGA ACGCCAGCGT GCGCTGCCGA ACGGTGCATA CAGGTGTCGT GTTGCGCAGC GGTCCGTTAT ATGGTACATA GTCTCCATAC CCGCCTGTTG CCGTCTGACT TCTGCAAGAC GCGCTGCTGG CTTTCTGGTG GGTGCCGCCC GAGAACAGCA ATGCGAAAAC GAAGCTCTCC GGTGACGTTG CGGACACCTC GGCGTGCACC GAATCTGGTC ATCGCCCATT GTTCGGCTTC GTCACGGCAA TATGTCCAGC AGTGTTCGCG GTTCGCTGGT GGCGAGGCTT GTTGTCGCAA CTACGCGTTC GCAGCCACCA TGGTCGCGTA GAAAGCCCAG TGTTACCCTG TACGACCGAC TACAACCACT GGGTACACCA TCCGTCCACC GTTGACCACA GCCGTCACCA TTACGACCGG ACACGTTTCC CTACTGGG
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