Suppose you want to determine whether a particulargene X is important for specification of the pharynx,but mutations in this same gene disrupt embryonicdevelopment well before pharyngeal structures appear.How could you use myo-2::GFP, the myo-2 promoter,the DNA sequence of gene X, and your knowledge ofRNA interference (RNAi) to generate worms that lackgene X expression in the pharynx but express gene Xin all other tissues in which it is expressed in wildtype C. elegans?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Suppose you want to determine whether a particular
gene X is important for specification of the pharynx,
but mutations in this same gene disrupt embryonic
development well before pharyngeal structures appear.
How could you use myo-2::GFP, the myo-2 promoter,
the DNA sequence of gene X, and your knowledge of
RNA interference (RNAi) to generate worms that lack
gene X expression in the pharynx but express gene X
in all other tissues in which it is expressed in wildtype C. elegans?

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