Strength 22. You are analyzing a new human neuronal enhancer. You cloned the enhancer in front of a minimal promoter, transfected a neuronal cell line, and quantified GFP expression. You then performed the deletion analysis shown on the right. Which letter designates the minimal region where enhancer activity is located? min. Novel enhancer prom. GFP of expression +++ +/- +++ +/- +++ a. a b. b C. C +++ +/- d. d e. both b and c are needed

Strength 22. You are analyzing a new human neuronal enhancer. You cloned the enhancer in front of a minimal promoter, transfected a neuronal cell line, and quantified GFP expression. You then performed the deletion analysis shown on the right. Which letter designates the minimal region where enhancer activity is located? min. Novel enhancer prom. GFP of expression +++ +/- +++ +/- +++ a. a b. b C. C +++ +/- d. d e. both b and c are needed

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Enhancer RPA Gene A Use the diagram above, which depicts a chromosomal region less than 500,000 bp, to determine the expression of each gene under the scenarios specified in the table. Regulatory promoters are indicated by "RP". Genes A, B, and C have intermediate expression when no regulatory proteins are bound. Complete the table by choosing high, intermediate, or low to describe the expression level of each gene under each specified scenario (i and ii). No regulatory proteins bound (i) A repressor binds to RPA and an activator binds to RPC (ii) A regulatory protein binds at each of the activator, insulator, and silencer. Expression of A? Intermediate 1. [Select] 4. [Select] Insulator RPB Expression of B? 2. Gene B Intermediate 5. [Select] [Select] Silencer RPC Expression of C? 3. Gene C Intermediate 6. [Select] [Select]
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From the list given - choose all the regulatory sequences that you think would control the expression of this eukaryotic gene THERE are multiple answer for this that I can choose Group of answer choices A 3' UTR B PPE sequences C 5' UTR D introns E core promoter F coding sequence G DNA-bending protein H enhancer sequence I mediator protein
Question: The beow are basics of transcription steps: Nucleosome remodeling to encourage transcriptional machinery to bind to a promoter. Pre-initiation that includes TFs binding to a core promoter sequence that recruit activators, repressors and RNA polymerase creating a pre-initiation complex. Initiation requires helicase activity to "unwind" the DNA. Promoter clearance involves the RNA polymerase unwinding and pulling DNA into the transcription complex. Consider that there is a high rate of abortion, which means promoter clearance does not occur and therefore transcription does not occur. Elongation occurs using one strand of the DNA in the 3' to 5' direction to produce a complimentary copy of the gene as a single stranded RNA molecule. Recall AT and GC associations. In RNA, the T is substituted with a U (uracil). Termination involves cleavage of the new RNA and the addition of adenines at the 3" end, which are important for translation as will be discussed in unit 4. 1.…
Cell and Molecular Biology Subject: 1. Explain how the actions of the lac operon are different from the trp operon. 2. Why is it important for a multicellular organism to be able to turn on only some of its genes in any particular tissue or cell type?
S-Cdk is likely to be a [Select] The inhibitor of Ras is likely to be a [Select] The MAP kinase is likely to be a [Select] Q Search 14:31 Protein X is a transcription factor that normally activates the expression of genes needed for S- phase. The activator of protein X is likely to be a [Select] is 4 ******* > 21**113 ***** ****** V f6 99+ 1- Il app.honorlock.com is sharing your screen. **** 1 a hp Stop sha
A bidirectional enhancer has the following sequence: 5′–GTCA–3′ 3′–CAGT–5′ Which of the following sequences would also be a functional enhancer? a. 5′–ACTG–3′ 3′–TGAC–5′ b. 5′–TGAC–3′ 3′–ACTG–5′ c. 3′–GTCA–5′ 5′–CAGT–3′ d. 3′–TGAC–5′ 5′–ACTG–3′
6 of 22 All cells in a given mammal carry the same genome, yet certain genes are expressed only in a particular tissue, such as in the eye, and not expressed in other tissues, such as in the liver. How is this specificity of gene expression for a particular tissue achieved? O All different cell types in the organism express all proteins equally and degrade the proteins that they don't need. O Different tissues express unique transcription factors that are bound to tissue-specific enhancer elements, thereby achieving tissue-specific gene expression. O Certain organ and tissue systems, such as the nervous system, form relatively early in the organism's embryonic life, as compared to the epidermis; it is this relative age of the tissue lineage that determines which tissue- specific genes are expressed. O Different tissues inactivate whole swaths of the genome, thereby achieving tissue-specific gene expression. O Each tissue has a unique RNA polymerase holoenzyme that achieves…
INSTRUCTION: = IF BOTH STATEMENT ARE TRUE = IF FIRST STATEMENT IS TRUE WHILE SECOND STATEMENT IS FALSE = IF FIRST STATEMENT IS FALSE WHILE SECOND STATEMENT IS TRUE = IF BOTH STATEMENTS ARE FALSE STAMENT 1: RNA splicing is the step in post transcriptional processing where intervening sequences are removed STAMENT 2: 5’ to 3’ direction is the direction of growth of the peptide chain ANSWER: STAMENT 1: The enzyme that joins the gaps in newly synthesized DNA is called DNA polymerase STAMENT 2: The name of the compound formed when cytosine is bonded to ribose is cytidine ANSWER: STAMENT 1: Codon is a term that refers to the 3-nucleotide code for amino acids in mRNA STAMENT 2: Transition is a kind of mutation where a purine changes to another purine ANSWER:
Time (h) 0 1 3 6 12 Mouse thymus cells were treated with 10 micromolar dexamethasone to induce apoptosis. At the indicated times the chromosomal DNA was extracted and analyzed by gel electrophoresis, which separates the DNA fragments based upon size differences. When cells undergo apoptosis their DNA is cut into fragments that are approximately multiples of 200 base pairs. This results in a characteristic “ladder" appearance when the fragments are separated by gel electrophoresis, as shown above. Based upon what you know about eukaryotic chromatin, explain why this pattern of DNA fragments is obtained. 6.
protein. You create a mouse line with Cas9 under control of a brain-specific enhancer, while the short guide RNA complementary to the first exon of Gene Y is expressed in all tissues. You subsequently sequence Gene Y in both brain and liver tissue. What would expect in each tissue? You can assume that the CRISPRICas9 system will impact both copies of Gene Y in cells, and that the first exon of Gene Y is necessary for Gene Ys function. a. Liver: Functional Gene Y; Brain: Functional Gene Y b. Liver: Nonfunctional Gene Y; Brain: Funtional Gene Y c. Liver: Functional Gene Y; Brain: Nonfunctional Gene Y d. Liver: Nonfunctional Gene Y; Brain: Nonfunctional Gene Y
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