A bidirectional enhancer has the following sequence: 5′–GTCA–3′ 3′–CAGT–5′ Which of the following sequences would also be a functional enhancer? a. 5′–ACTG–3′ 3′–TGAC–5′ b. 5′–TGAC–3′ 3′–ACTG–5′ c. 3′–GTCA–5′ 5′–CAGT–3′ d. 3′–TGAC–5′ 5′–ACTG–3′
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A bidirectional enhancer has the following sequence:
5′–GTCA–3′ 3′–CAGT–5′ Which of the following sequences would also be a functional enhancer?
a. 5′–ACTG–3′ 3′–TGAC–5′
b. 5′–TGAC–3′ 3′–ACTG–5′
c. 3′–GTCA–5′ 5′–CAGT–3′
d. 3′–TGAC–5′ 5′–ACTG–3′
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- An enhancer, located upstream from a gene, has the following sequence: 5′–GTAG–3′ 3′–CATC–5′ This enhancer is orientation-independent. Which of the following sequences also works as an enhancer? A. 5′–CTAC–3′ 3′–GATG–5′ B. 5′–GATG–3′ 3′–CTAC–5′ C. 5′–CATC–3′ 3′–GTAG–5′ C15.An enhancer, located upstream from a gene, has the followingsequence: 5´–GTAG–3´ 3´–CATC–5´This enhancer is orientation-independent. Which of the followingsequences also works as an enhancer?A. 5´–CTAC–3´ 3´–GATG–5´B. 5´–GATG–3´ 3´–CTAC–5´C. 5´–CATC–3´ 3´–GTAG–5´An enhancer, located upstream from a gene, has the following sequence: 5’-ACGA-3’ 3’-TGCT-5’ This enhancer is orientation-independent. Which of the following sequences also works as an enhancer? Why? a. 5’-TGCT-3’ 3’-ACGA-5’ b. 5’-TCGT-3’ 3’-AGCA-5’
- Through alternative splicing, eukaryotes (a) reinforce gene inactivation (b) prevent transcription of heterochromatin (c) produce related but different proteins in different tissues (d) amplify genes to meet the requirement of high levels of a gene product (e) bind transcription factors to enhancers to activate transcriptione In the figure below, what is represented by the line labeled A? TFIIB recognition element DNA in m 3' G/CGICGIC CGCC -35 TATA box TATAAA -25 A Initiator element YYAN TAYY +1 Downstream core promoter element RGATCGTG +30iestiðn Pre-initiation complex (PIC) is formed right before the initiation of genes, which of the following steps seemes to NOT help for the PIC formation? O TBP protein to recognize TATA box at promoter region O TFIIA to recognize the TAFS in TFIID O Enhancers to attract coactivators and activators O Silencers to attract repressor and corepressors
- An enhancer, located upstream from a gene, has the following sequence: 5’ – GTAG – 3' 3'- САТС- 5' The enhancer is orientation-independent. Which of the following sequences also work(s) as enhancer? Why not others? (Only the top strand is shown; the sequence and polarity of the bottom strand can be inferred.) a. 5'- СТАС -3' b. 5' – GATG – 3' с. 5'— САТС-3' с. 5' — GTTC - 3'In which strand is the promoter? 5' TCATGAGATA GCCATGCATA TCTAGTATGT AGGCATCTGA GTTTATATCT CA 3’ 3' AGTACTCTAT CGGTACGTAT AGATCTATCA TCCGTAGACT CAAATATAGA GT 5'a. Use circles for activators and squares for repressors. Draw the shapes bound to each promoter region. b. Under the word gene write “ON” or “OFF” to indicate if the gene is transcribed or not under the different conditions.
- 5 5 S 6 5 5 5 6 U 6 U 6 5:14 PM | 0.2KB/s HHHHH R R U RUUR ARU AP AP R U U R R AP R R R AP MOLECULAR...GENETICS. Describe gene regulation at transcription level. Explain the role of antsense RNA in control mechanism. Describe translational control mechanisms. Describe common DNA damages. Distinguish excision and mismatch repair. Describe the role of recA protein in recombination repair Elaborate on SOS repair mechanism. Define thymine dimer. How are they formed and repaired? Describe the molecular basis of mutation. 11 Leu+ Met+ Arg+ Write a detailed note on spontaneous mutation. Explain about mutant detection methods. Define reverse mutation. Describe the mechanism underlying Intragenic and intergenic suppressor mutations Describe the transposition mechanisms. 13 Vo LTE UNIT IV Time (Min) Describe the process of generalised transformation occurring in bacterial chromosome and plasmid. Elaborate on molecular mechanism and significance of transformation 22 Describe the process of…Identify the labeled factors in the figure below (A and B) and indicate the direction of transcription. ts A O A= Mediator, B= TFIID; direction is into the screen O A= TEIIE, B= TFIIH; direction is into the screen rences A= TBP, B= TEIIB; direction is out of the screen O A= TFIIF, B= TFIIE; direction is out of the screenExplain in detail the role of super-enhancers. Give some examples. How the super-enhancers can be identified?