protein Menchie is conserved from cats to humans. The region upstream of the menchie gene has three conserved sequences: region A, B, and C. Mutations were made in this upstream region by removing these regions or changing the orientation of these regions. Additionally, the coding sequence for Menchie was replaced with the coding sequence for green fluorescent protein (GFP; gene = gfp). GFP fluorescence was measured for each of the indicated constructs and the results are summarized. The arrow indicates the direction of transcription and where transcription starts (the +1 site). a. What is the purpose
The protein Menchie is conserved from cats to humans. The region upstream of the menchie gene has three conserved sequences: region A, B, and C. Mutations were made in this upstream region by removing these regions or changing the orientation of these regions. Additionally, the coding sequence for Menchie was replaced with the coding sequence for green fluorescent protein (GFP; gene = gfp). GFP fluorescence was measured for each of the indicated constructs and the results are summarized. The arrow indicates the direction of transcription and where transcription starts (the +1 site).
a. What is the purpose of replacing the coding sequence of Menchie with the coding sequence of GFP?
b. Which region (A, B, or C) contains the promoter sequence? Briefly explain your answer, using at least two lines of evidence from the results shown; please use the #s to indicate the line of evidence you are describing.
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