The protein Menchie is conserved from cats to humans. The region upstream of the menchie gene has three conserved sequences: region A, B, and C. Mutations were made in this upstream region by removing these regions or changing the orientation of these regions. Additionally, the coding sequence for Menchie was replaced with the coding sequence for green fluorescent protein (GFP; gene = gfp). GFP fluorescence was measured for each of the indicated constructs and the results are summarized. The arrow indicates the direction of transcription and where transcription starts (the +1 site). c. Which region (A, B, or C) contains an enhancer sequence? Briefly explain your answer, using at least two lines of evidence from the results shown; please use the #s to indicate the line of evidence you are describing. d. Which region (A, B, or C) contains a silencer sequence? Briefly explain your answer, using at least two lines of evidence from the results shown; please use the #s to indicate the line of evidence you are describing.
Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
The protein Menchie is conserved from cats to humans. The region upstream of the menchie gene has three conserved sequences: region A, B, and C. Mutations were made in this upstream region by removing these regions or changing the orientation of these regions. Additionally, the coding sequence for Menchie was replaced with the coding sequence for green fluorescent protein (GFP; gene = gfp). GFP fluorescence was measured for each of the indicated constructs and the results are summarized. The arrow indicates the direction of transcription and where transcription starts (the +1 site).
c. Which region (A, B, or C) contains an enhancer sequence? Briefly explain your answer, using at least two lines of evidence from the results shown; please use the #s to indicate the line of evidence you are describing.
d. Which region (A, B, or C) contains a silencer sequence? Briefly explain your answer, using at least two lines of evidence from the results shown; please use the #s to indicate the line of evidence you are describing.
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