If the EcoRI/HindIII (stuffer) fragment cut from the vector is religated back into the vector, the M13F/M13R primers will: O Amplify the stuffer fragment because the primers are designed to complement the plasmid DNA O Not amplify the stuffer fragment because the primers are designed to complement human gDNA The primers you used to PCR amplify your human genomic DNA insert from your bacterial colony were designed to complement (or bind): O plasmid pUC19 O human genomic DNA O your mtDNA O All of the above OC

If the EcoRI/HindIII (stuffer) fragment cut from the vector is religated back into the vector, the M13F/M13R primers will: O Amplify the stuffer fragment because the primers are designed to complement the plasmid DNA O Not amplify the stuffer fragment because the primers are designed to complement human gDNA The primers you used to PCR amplify your human genomic DNA insert from your bacterial colony were designed to complement (or bind): O plasmid pUC19 O human genomic DNA O your mtDNA O All of the above OC