If the EcoRI/HindIII (stuffer) fragment cut from the vector is religated back into the vector, the M13F/M13R primers will: O Amplify the stuffer fragment because the primers are designed to complement the plasmid DNA O Not amplify the stuffer fragment because the primers are designed to complement human gDNA The primers you used to PCR amplify your human genomic DNA insert from your bacterial colony were designed to complement (or bind): O plasmid pUC19 O human genomic DNA O your mtDNA O All of the above OC
If the EcoRI/HindIII (stuffer) fragment cut from the vector is religated back into the vector, the M13F/M13R primers will: O Amplify the stuffer fragment because the primers are designed to complement the plasmid DNA O Not amplify the stuffer fragment because the primers are designed to complement human gDNA The primers you used to PCR amplify your human genomic DNA insert from your bacterial colony were designed to complement (or bind): O plasmid pUC19 O human genomic DNA O your mtDNA O All of the above OC
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:If the EcoRI/HindIII (stuffer) fragment cut from the vector is religated back into the vector, the M13F/M13R primers will:
O Amplify the stuffer fragment because the primers are designed to complement the plasmid DNA
O Not amplify the stuffer fragment because the primers are designed to complement human gDNA
Transcribed Image Text:The primers you used to PCR amplify your human genomic DNA insert from your bacterial colony were designed to complement (or bind):
O plasmid pUC19
O human genomic DNA
O your mtDNA
O All of the above
OC
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