A DNA fragment of interest is inserted in the BamHI site of plasmid pBR322.The recombinants will be: HindlII EcoRI EcoRV 1/ BạmHI Sall Pstl, tet аmp noe PBR322 4361 bp 3000 ori 22 Ndel O A. Resistant to amp an tet O B. None of the other options, Xgal is required for selection O C. xensitive to amp, resistant to tet None of the other options, Xgal is required for selection O D. Sensitive to tet, resistant amp O E. Sensitive to amp and tet

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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A DNA fragment of interest is inserted in the BamHI site of plasmid PBR322.The recombinants will be:
HindIII
ECORI
EcoRV
BamHI
439 0 29 is
4000
375
Sall
PstI,
651
tet
amp
1000
PBR322
4361 bp
3000
ori
2000
2295
Ndel
A. Resistant to amp an tet
B. None of the other options, Xgal is required for selection
O C. xensitive to amp, resistant to tet
None of the other options, Xgal is required for selection
O D. Sensitive to tet, resistant amp
O E. Sensitive to amp and tet
Transcribed Image Text:A DNA fragment of interest is inserted in the BamHI site of plasmid PBR322.The recombinants will be: HindIII ECORI EcoRV BamHI 439 0 29 is 4000 375 Sall PstI, 651 tet amp 1000 PBR322 4361 bp 3000 ori 2000 2295 Ndel A. Resistant to amp an tet B. None of the other options, Xgal is required for selection O C. xensitive to amp, resistant to tet None of the other options, Xgal is required for selection O D. Sensitive to tet, resistant amp O E. Sensitive to amp and tet
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