10. V(D)J recombination contributes to the diversity of immunoglobulins, and occurs by: A. Random single-strand nicks in the DNA B. Programmed double-strand breaks in the DNA C. Site-specific recombination D. Homologous recombination E. Retrotransposition 11. Zsuzanna Izsvak and coworkers set out to make a functional copy of a nonfunctional transposase in a fish that is estimated to have been nonfunctional for over 14 million years. The functional transposase became known as ____________________. A. Sleeping Beauty B. Rumpelstiltskin C. Pandora D. Rip van Winkle E. None of the above 12. Which of the following Tn3 proteins catalyzes recombination to separate the donor and acceptor DNA molecules? A. transposase B. DNA polymerase C. RecA D. Resolvase E. β-lactamase
Genetic Recombination
Recombination is crucial to this process because it allows genes to be reassorted into diverse combinations. Genetic recombination is the process of combining genetic components from two different origins into a single unit. In prokaryotes, genetic recombination takes place by the unilateral transfer of deoxyribonucleic acid. It includes transduction, transformation, and conjugation. The genetic exchange occurring between homologous deoxyribonucleic acid sequences (DNA) from two different sources is termed general recombination. For this to happen, an identical sequence of the two recombining molecules is required. The process of genetic exchange which occurs in eukaryotes during sexual reproduction such as meiosis is an example of this type of genetic recombination.
Microbial Genetics
Genes are the functional units of heredity. They transfer characteristic information from parents to the offspring.
10. V(D)J recombination contributes to the diversity of immunoglobulins, and occurs by:
A. Random single-strand nicks in the DNA
B. Programmed double-strand breaks in the DNA
C. Site-specific recombination
D. Homologous recombination
E. Retrotransposition
11. Zsuzanna Izsvak and coworkers set out to make a functional copy of a nonfunctional transposase in a fish that is estimated to have been nonfunctional for over 14 million years. The functional transposase became known as ____________________.
A. Sleeping Beauty
B. Rumpelstiltskin
C. Pandora
D. Rip van Winkle
E. None of the above
12. Which of the following Tn3 proteins catalyzes recombination to separate the donor and acceptor DNA molecules?
A. transposase
B. DNA polymerase
C. RecA
D. Resolvase
E. β-lactamase
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