4. The bars in the following sequence indicate the breakpoints of a deletion. 5'-CGGGTATCTACTAAA|TTCGCACTTACGAGGATTAACATCCGATTG|TACCGAATGAGAATC-3' Which primer pair would you use to genotype for this deletion, such that all genotypes will result in a band? a. 5'-CGGGTATCTACTAAA-3' and 5'-TACCGAATGAGAATC-3' b. 5'-CGGGTATCTACTAAA-3' and 5'-GATTCTCATTCGGTA-3' с. 5'-CGGGTAТСТАСТААА-З" and 5'-CCТCGTAAGTGCGAA-3' d. 5'-CGGGTATCTACTAAA-3' and 5'-CATCCGATTGTACCG-3' e. There is no way to design a pair of primers that provide positive evidence

Human Anatomy & Physiology (11th Edition)
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Chapter1: The Human Body: An Orientation
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4. The bars in the following sequence indicate the breakpoints of a deletion.
5'-CGGGTATCTACTAAA|TTCGCACTTACGAGGATTAACATCCGATTG|TACCGAATGAGAATC-3'
Which primer pair would you use to genotype for this deletion, such that all genotypes will result in a
band?
a. 5'-CGGGTATCTACTAAA-3' and 5'-TACCGAATGAGAATC-3'
b. 5'-CGGGTATCTACTAAA-3' and 5'-GATTCTCATTCGGTA-3'
с. 5'-CGGGTAТСТАСТААА-З" and 5'-CCТCGTAAGTGCGAA-3'
d. 5'-CGGGTATCTACTAAA-3' and 5'-CATCCGATTGTACCG-3'
e. There is no way to design a pair of primers that provide positive evidence
Transcribed Image Text:4. The bars in the following sequence indicate the breakpoints of a deletion. 5'-CGGGTATCTACTAAA|TTCGCACTTACGAGGATTAACATCCGATTG|TACCGAATGAGAATC-3' Which primer pair would you use to genotype for this deletion, such that all genotypes will result in a band? a. 5'-CGGGTATCTACTAAA-3' and 5'-TACCGAATGAGAATC-3' b. 5'-CGGGTATCTACTAAA-3' and 5'-GATTCTCATTCGGTA-3' с. 5'-CGGGTAТСТАСТААА-З" and 5'-CCТCGTAAGTGCGAA-3' d. 5'-CGGGTATCTACTAAA-3' and 5'-CATCCGATTGTACCG-3' e. There is no way to design a pair of primers that provide positive evidence
Expert Solution
Step 1

The given sequence has bars indicating the breakpoint of a deletion means we are going to delete the sequence that is in the middle of bars. For which we have to design a pair of primers.

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