8) Draw a two-stranded, antiparallel ß-sheet with at least four amino acids in each B-strand.

Answered: Image /qna-images/answer/c70e577b-6ea8-46de-bb61-bb27fc8850a9.jpg

Answered: 8) Draw a two-stranded, antiparallel…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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E. coli cells are simultaneously infected with two strains of phage λ. One strain has a mutant host range, is temperature sensitive, and produces clear plaques (genotype h st c); another strain carries the wildtype alleles (genotype h+ st+ c+). Progeny phages are collected from the lysed cells and are plated on bacteria. The following numbers of different progeny phages are obtained: Progeny phage genotype Number of plaques h+ c+ st+ 321 h c st 338 h+ c st 26 h c+ st+ 30 h+ c st+ 106 h c+ st 110 h+ c+ st 5 h c st+ 6 a. Determine the order of the three genes on the phage chromosome. b. Determine the map distances between the genes. c. Determine the coefficient of coincidence and the interference
20 16. Which of the following methods may introduce foreign DNA into a recipient? A) transduction B) conjugation C) transformation D) All of the above 17. F+ strains of Escherichia coli A) have an integrated F factor. B) have the F factor as a plasmid. C) do not have an F factor. D) transfer the F factor to recipient cells at a high frequency. 18. What is the function of the conjugation pilus? A) It contains an F plasmid. B) It carries the chromosome of F- cells. C) It converts F- cells into F+ cells. D) It pulls the F+ and F- cells together. 19. When a transposon is cut from one location in genome and pasted into a new location, it is called replicative transposition A) True B) False (0 20. Insertion sequences are bounded by inverted repeats and encode for transposase. A) True B) False 21. The CRISPR system UM (8 A) recognizes foreign DNA sequences that have previously entered the cell and directs the Cas proteins to destroy them. B) synthesizes gene transfer agents during stationary…
Briefly describe the two approaches a bacteriophagecan take to trigger an infection once inside its host cell.
Briefly describe the lytic and lysogenic cycles of bacteriophages.In your answer, explain what a prophage is?
During the [X] phase of CRISPR-Cas9 defense, sequences from a new bacteriophage that infects the cell are chopped by a Cas1/Cas2 complex and integrated into the CRISPR locus, >A Moving to another question will save this response.
Bacteria exposed to viruses incorporate sections of the virus’s DNA into the CRISPR array sequences in their genome. This mechanism allows bacteria to fight off the viruses, like an immune response: the information in CRISPR spacers served as “coordinates” for recognizing and cutting up invading DNA sequences. Describe what might happen under the conditions described after a bacteriophage infects a bacterial cell and releases its DNA into the bacterial cell. Explain why: The cas genes on the bacterial genome contains a frameshift deletion mutation that alters the function of the protein The bacteria will be unable to elicit an immune response and will succumb to the phase infection
The genotypes of five merozygote bacteria are shown below. In each set, the genotype of the lac operon on the chromosome is on the left side of the slash () and the genotype of the lac operon on the F' plasmid is on the right side of the slash. Which of the merozygote bacteria listed below would be able to make high quantities of B-galactosidase in the absence of lactose? Mark all the answers that are correct. O i p*o°z¯ y*A*/F'I$ p*oʻz*y*A* I* p* o*Z¯YA*/F'T P*O*z*y* A* I* P* o*z*Y* A* /F'T P*O*z*y* A* O 1* p*o*z° YA*/F'T P*o°z*y*A* 1S p*0°2¯Y¯AZET P*O*Z*Y*A*
What is required for a bacteriophage T4 virion toattach to an Escherichia coli cell?
Name a nucleoside analog. Explain how that nucleoside analog works. Which viruses are effectively treated by that nucleoside analog?
One of the reasons why phage therapy has not been applied widely is that bacteria can become resistant to bacteriophages as well, through mutations in genes encoding for specific proteins. What would be a protein in the bacterial cell that, if mutated, would make that cell resistant to phage infection?
What is the “life” cycle of a T-even bacteriophage?
What evolutionary advantage would a retrovirus gain by having the ability to regulate the sites of splicing of its RNA?

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