1. Determine the amount of Ca(OH)2 that is required to prepare a 300 ml of a 2N Ca(OH)2 solution. [Molar mass Ca(OH)2 = 74 g/mole] 2. 10 μM is equivalent to? in nanomol/µL , millimol/µL, micromol/μL, and picomol/µL 3. The final concentration of each dNTP required in a PCR reaction is 150 μM. If the total volume of the PCR reaction is equal to 50 µl and the stock concentration of each primer in the dNTP mix is equal to 1.5 mM. Calculate the volume of stock dNTP mix which needs to be added in order to ensure a dNTP concentration of 150 μM.
DNA and RNA
Deoxyribonucleic acid (DNA) is usually called the blueprint of life. Deoxyribose is a monosaccharide that has a key function in the synthesis of deoxyribonucleic acid. One less oxygen-containing hydroxyl group occurs in deoxyribose sugar. Nucleic acid, deoxyribonucleic acid, is one of the natural components. Deoxyribonucleic acid is a double-stranded molecule. Watson and Crick postulated the double-stranded model of the helix. A deoxyribonucleic acid is a molecular group that carries and transmits genetic information from parents to offspring. All eukaryotic and prokaryotic cells are involved.
DNA as the Genetic Material
DNA, or deoxyribonucleic acid, is a long polymeric nucleic acid molecule discovered in the late 1930s. It is a polymer; a long chain-like molecule made up of several monomers connected in a sequence. It possesses certain characteristics that qualify it as a genetic component. Certain organisms have different types of nucleic acids as their genetic material - DNA or RNA.
Genetics
The significant branch in science which involves the study of genes, gene variations, and the organism's heredity is known as genetics. It is also used to study the involvement of a gene or set of genes in the health of an individual and how it prevents several diseases in a human being. Thus, genetics also creates an understanding of various medical conditions.
DNA Replication
The mechanism by which deoxyribonucleic acid (DNA) is capable of producing an exact copy of its own is defined as DNA replication. The DNA molecules utilize a semiconservative method for replication.
1. Determine the amount of Ca(OH)2 that is required to prepare a 300 ml of a 2N Ca(OH)2 solution. [Molar mass Ca(OH)2 = 74 g/mole]
2. 10 μM is equivalent to? in nanomol/µL , millimol/µL, micromol/μL, and picomol/µL
3. The final concentration of each dNTP required in a PCR reaction is 150 μM. If the total volume of the PCR reaction is equal to 50 µl and the stock concentration of each primer in the dNTP mix is equal to 1.5 mM. Calculate the volume of stock dNTP mix which needs to be added in order to ensure a dNTP concentration of 150 μM.
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