II. Making your Master Mix and PCR Set up for TPCN2: Note: the reaction volumes will be 20 µL and n = 10 reactions! Assume you extract gDNA quantitated by the NanoDrop at a concentration of 1065.7 ng/μL. Calculate the dilution(s) needed to get to a concentration of 25 ng/μL needed for making your Master Mix. Show pipettable volumes! Do two-step dilution.... Show work.

II. Making your Master Mix and PCR Set up for TPCN2: Note: the reaction volumes will be 20 µL and n = 10 reactions! Assume you extract gDNA quantitated by the NanoDrop at a concentration of 1065.7 ng/μL. Calculate the dilution(s) needed to get to a concentration of 25 ng/μL needed for making your Master Mix. Show pipettable volumes! Do two-step dilution.... Show work.

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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