II. Making your Master Mix and PCR Set up for TPCN2: Note: the reaction volumes will be 20 µL and n = 10 reactions! Assume you extract gDNA quantitated by the NanoDrop at a concentration of 1065.7 ng/μL. Calculate the dilution(s) needed to get to a concentration of 25 ng/μL needed for making your Master Mix. Show pipettable volumes! Do two-step dilution.... Show work.

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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**II. Making your Master Mix and PCR Set up for TPCN2:**

**Note: the reaction volumes will be 20 μL and n = 10 reactions!**

Assume you extract gDNA quantitated by the NanoDrop at a concentration of 1065.7 ng/μL. Calculate the dilution(s) needed to get to a concentration of 25 ng/μL needed for making your Master Mix. Show pipettable volumes! Do two-step dilution... Show work.
Transcribed Image Text:**II. Making your Master Mix and PCR Set up for TPCN2:** **Note: the reaction volumes will be 20 μL and n = 10 reactions!** Assume you extract gDNA quantitated by the NanoDrop at a concentration of 1065.7 ng/μL. Calculate the dilution(s) needed to get to a concentration of 25 ng/μL needed for making your Master Mix. Show pipettable volumes! Do two-step dilution... Show work.
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