1. From one colony of E.coli, you have purified the DNA of a recombinant plasmid. You have resuspended the DNA in a volume of 150 uL. You dilute 15 µL of the purified DNA sample into a total volume of 300 μL TE buffer. You measure the absorbance of this diluted sample at 260 and 280 nm and obtain the following readings: 4260=0.457; 4280 = 0.243 NOTE answer B QUESTION ONLY Write a complete computation: a) What is the DNA concentration of the 150 µL plasmid prep? b) How much total DNA (in ug) was purified by the plasmid prep procedure?
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
answer B only
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