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29. You have purified plasmid DNA and would like to determine its concentration. You mix 10 µL of DNA into 990 µl of TE buffer and read the absorbance in a quartz cuvette at 260 nm and 280 nm. The absorbance values are as follows: Aaso = 1.625 A2s0 = 0.864 a) Calculate the concentration of the DNA sample b) Given the information above, how could you test the purity of this sample? Is the DNA pure? If not, what are the likely contaminants?

29. You have purified plasmid DNA and would like to determine its concentration. You mix 10 µL of DNA into 990 µl of TE buffer and read the absorbance in a quartz cuvette at 260 nm and 280 nm. The absorbance values are as follows: Aaso = 1.625 A2s0 = 0.864 a) Calculate the concentration of the DNA sample b) Given the information above, how could you test the purity of this sample? Is the DNA pure? If not, what are the likely contaminants?

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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29. You have purified plasmid DNA and would like to determine its concentration. You mix 10 µL of DNA into 990 µl of TE buffer and read the absorbance in a quartz cuvette at 260 nm and 280 nm. The absorbance values are as follows: Azs0 = 1.625 A280 = 0.864 a) Calculate the concentration of the DNA sample b) Given the information above, how could you test the purity of this sample? Is the DNA pure? If not, what are the likely contaminants?
Transcribed Image Text:29. You have purified plasmid DNA and would like to determine its concentration. You mix 10 µL of DNA into 990 µl of TE buffer and read the absorbance in a quartz cuvette at 260 nm and 280 nm. The absorbance values are as follows: Azs0 = 1.625 A280 = 0.864 a) Calculate the concentration of the DNA sample b) Given the information above, how could you test the purity of this sample? Is the DNA pure? If not, what are the likely contaminants?
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