1. At what ratio of A260/280 can we say that DNA is pure? What about RNA and protein? 2. While spectrophotometric methods are effective at detecting DNA, a more sensitive but  expensive technique called fluorometry is used in sensitive applications. What is the principle  behind fluorometry and why is it better than spectrophotometry in detecting DNA?

Q: With regard to the experiment described in Figure, The DNA extract was treated with DNase, RNase, or…

A: Mutation is defined as the sudden inheritable change that occurs in the DNA sequence.  It is caused…

Q: Calculate the volume (in µL) of DNA template required to add 60.0 ng to a 20.0 µL PCR if the DNA…

A: Concentration is the term used to define the amount of a specific entity with respect to the total…

Q: Reagents Sterile ddH₂0 10X PCR Buffer (E) mM MgCl₂ 10mM dNTP (1) mM Forward Primer 50 mM Reverse…

A: Polymerase chain reaction (abbreviated PCR) is a laboratory technique for rapidly producing…

Q: a) Based on your calculations of the DNA concentrations, how much DNA was loaded into each well? Do…

A: DNA yeild is measured on basis of absorbance.  At A260 absorbance DNA absorbs light most strongly,…

Q: a PCR-based crime scene investigation, similar to the one presented in the lab module with Brother Y…

A: DNA sample taken from a crime scene is compared with a DNA sample from a suspect. If the two DNA…

Q: A student is trying to add 15.0 ng of DNA template to a 20.0 µL PCR.  The DNA template is at a…

A: Polymerase Chain Reaction or PCR is a method to make multiple copies of DNA starting from a small…

Q: Protocols in isolating DNA often involve the use of two kinds of ethanol, 100% ethanol and  70%…

A: 70% ethanol is used in washing DNA furing DNA extraction. 100% ethanol is used in the final step. In…

Q: researcher runs an agarose gel IIl ladder one läñé ând 100 ng of a 500 bp DNA fragment in another…

A: Agarose gel electrophoresis or AGE is a form of gel electrophoresis that is used for discriminate…

Q: (Practice Hint: Click on the right-hand column to choose a bar height. Click again to change the bar…

A: DNA is made up of four nitrogenous bases: adenine (A), thymine (T), guanine (G), and cytosine (C).…

Q: After properly troubleshooting, you finally are able to visualize the gel. You obtained the image…

A: DNA extraction is a protocol of isolating genomic DNA from any cell type. Generally we use the…

Q: How would you solve this question? I am confused

A: The objective of this question is to estimate the size of an unknown DNA fragment using a standard…

Q: What are the benefits of using a mixture classification scheme as outlined in DNA Box 14.1? What…

A: INTRODUCTION German stain commission This commission includes several forensic science and legal…

Q: 2) A scientist wants to increase the amount of sample of a DNA sequence of 100 bps. Calculate the…

A: Given that molar mass of average DNA base pair is 600g/mol. That means 600g of base pairs contain…

Q: Please answer the following using the experiment data below. Provide the formulas or methods used…

A: Transformation efficiency is defined as the number of colonies obtained per microgram of DNA plated.

Q: Which of the following is true regarding next generation sequencing? 1. Next generation sequencing…

A: Answer Next generation sequencing  is a powerful platform that has enabled the sequencing of…

Q: Scenario 1: DNA concentration? UV-Vis spectroscopy [Choose ] Homology Modeling Scenario 2: Does it…

A: Different techniques are used for determining different properties.

Q: 1. Why do we need to check the isolated DNA for its quantity and quality? 2. The purity of DNA…

A: Deoxyribonucleic acid DNA isolation is referred to the extraction process of DNA from different…

Q: A 1% by weight agarose gel is typically used in agarose gel electrophoresis. If you used a 2%…

A: During agarose gel electrophoresis, an agarose solution is prepared, it is cooled to prepare a gel,…

Q: In order to determine the purity of a DNA sample, spectrophotometry can be carried our at Answer to…

A: Spectrophotometry is the quantitative analysis of spectra to compare the relative absorption or…

Q: Match each statement with the best possible single answer from the list below. Terms may be used in…

A:

Q: In a PCR-based crime scene investigation, similar to the one presented in the lab module with…

A: The RAPD or rapid amplification of polymorphic DNA is used to detect the DNA that is present in the…

Q: How would you solve this and how would it look? i am confused

A: The objective of this question is to estimate the size of an unknown DNA fragment using a standard…

Q: Sanger DNA sequencing/ dideoxy sequencing was used as shown in the diagram below. The arrow…

A: Sanger's sequencing method. It is a method based on DNA synthesis. In normal DNA synthesis,…

Q: During gel electrophoresis, in what direction (toward which electrode) does DNA move toward?…

A: There are different molecular biology tools and techniques used to analyze and quantify…

Q: To generate large amounts of DNA to manufacture gene therapy payloads or to be able to see them on…

A: Specific sequences can be amplified by PCR in order to generate large amounts of DNA to manufacture…

Q: Questions are talking about amplification of DNA When we run the gel, we also run a ladder why?

A: A DNA ladder is the mixture of amplified DNA fragments of different size range which is used as…

Q: Similarities between DNA agarose gels and SDS-PAGE are:

A: Gel electrophoresis is the technique of separation of molecules of the basis of their size and…

Q: To be able to properly analyze a sample of DNA, you need to have at least 1 µg of the DNA of…

A: The polymerase chain reaction (PCR) is a molecular biology technique for amplifying particular…

Q: Please explain

A: Lane A:* DNA sample: Human genomic DNA* Enzyme: EcoRI (5' G^AATTC 3')Lane B:* DNA sample:…

Q: ns: In each box, type the hrst letter of the base that correctly matches the given DNA sequences C…

A: Answer. A nucleotide is the monomeric unit of nucleic acids. Nucleic acids are therefore also called…

Q: Answers to the following questions. A. You are given a tube containing 275 ng of purified PCR…

A: 5) A. Amount of purified PCR product DNA in the tube = 275 ng = 275 000 pg (1000 pg = 1 ng) The…

Q: Could you please explain these:   1. Why analyze the unknowns in a (short) serial dilution rather…

A: Introduction: DNA concentration refers to the amount of DNA present in a sample. It is typically…

Q: please solve this with step-by-step calculations and explanations.

A: 1:

Q: Scene Electrophoresis o the questions below, including a labeled picture of you your gel, you had to…

A:

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.