Suppose you're going to do 9 PCR reactions. The starting and final concentrations of the ingredients are listed below. Each reaction should have a volume of 50 μl. Component, stock concentration 5X Reaction Buffer MgCl2, 25 mM Nucleotide Mix, 20mM Primer 1, 10 μM Primer 2, 10 μM Taq DNA Polymerase, 5 Units/μl Template DNA, concentration unknown Final Concentration or amount 1.0X 1.5 mM 0.2 mM 1.0 μΜ 1.0 ΜΜ 1.0 Units use 1 μl per reaction (a) Briefly explain how you would set up the reactions and show all your calculations. Indicate the amounts that you would pipet into your tubes. Be clear. (Show ALL your calculations on a piece of paper, take a picture and paste it in the space below). (b) Using the amounts listed above, how many pmol of each primer will there be in each reaction? (Show ALL your calculations on a piece of paper, take a picture and paste it in the space below).

Suppose you're going to do 9 PCR reactions. The starting and final concentrations of the ingredients are listed below. Each reaction should have a volume of 50 μl. Component, stock concentration 5X Reaction Buffer MgCl2, 25 mM Nucleotide Mix, 20mM Primer 1, 10 μM Primer 2, 10 μM Taq DNA Polymerase, 5 Units/μl Template DNA, concentration unknown Final Concentration or amount 1.0X 1.5 mM 0.2 mM 1.0 μΜ 1.0 ΜΜ 1.0 Units use 1 μl per reaction (a) Briefly explain how you would set up the reactions and show all your calculations. Indicate the amounts that you would pipet into your tubes. Be clear. (Show ALL your calculations on a piece of paper, take a picture and paste it in the space below). (b) Using the amounts listed above, how many pmol of each primer will there be in each reaction? (Show ALL your calculations on a piece of paper, take a picture and paste it in the space below).

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

See similar textbooks

Similar questions

In a controlled experiment, all of the variables are kept the same in order to be consistent. There is only one variable that is changed for these kinds of studies, and it is known as the __________ ___________ (2 words).
Second question please
Note:- Do not provide handwritten solution. Maintain accuracy and quality in your answer. Take care of plagiarism. Answer completely. You will get up vote for sure
For the following research question: In communities with high rates of opioid addiction (P), does the establishment of supervised injection sites (I) compared to the absence of such facilities (C) lead to a reduction in fatal and non-fatal opioid overdose cases (O)? How can I use these research articles to write An introduction which includes (opening statement, thesis statement, summary of paper) A one-page appendix with a Prisma literature search graphic depicting the search process and results, not sure how to do a Prisma at all. A concisely worded descriptive synthesis of the evidence regarding the topic of inquiry and a discussion of the implications for nursing practice, education, and research. (Synthesis will vary widely based on individual articles but should include the following elements; discussion/comparison of the study methods and populations. Commentary on the quality of the studies. Study findings are aggregated, discussed, compared/contrasted as appropriate. The…
Question 65SavedWhich of the following observations gives support to the ‘cytokine dysregulation’ theory of causation of depression? Question 65 options: Depression is common in infectious and autoimmune diseases Exposure of the CNS to cytokines induces depressive symptoms Some antidepressants have anti-inflammatory properties All of the above support a role for cytokines in depression
Please summarize these papers and explain what the figures mean. Please elaborate as if your explaining the class what this paper is about. Please explain what the figure tells us and what they do.
Question 83 What information would be appropriate when health teaching a client who is taking Beclovent (Beclomethasone)? Select all that apply. Question 83 options: Rinse your mouth after use to prevent oral fungal infections You need to have blood work done regularly You should always keep it with you It will be your primary drug for asthma control Drink a lot of fluids to prevent constipation
Question 1 Total claims denied: $10,000 Total claims submitted: $100,000 Time period: 3 months What's the claims denial rate?