Figure 3 16 Mean 14 level 12 of expres 10 sion strain 17 strain 22 17 (arbitr ary units) 31 28 11 21 Amount of recombinant DNA introduced Figure 3. Two engineered E. coli strains were made such that different variations of each strain had different numbers/amounts of recombinant DNA introduced for genes involved in Taxadiene synthesis (a precursor for Taxol). These strains were grown and then the amount of. a protein product in the Taxadiene pathway was measured. The data points are the mean values of protein product expression +/- standard deviation from four replicates 1. For the graph: a) Describe the graph: b) Describe the data: c) Interpret the data: Describe the statistics that are shown and what you can infer from them. Are there other statistics that would be better suited for this data? Is there any flaw in the way the data is represented?
Figure 3 16 Mean 14 level 12 of expres 10 sion strain 17 strain 22 17 (arbitr ary units) 31 28 11 21 Amount of recombinant DNA introduced Figure 3. Two engineered E. coli strains were made such that different variations of each strain had different numbers/amounts of recombinant DNA introduced for genes involved in Taxadiene synthesis (a precursor for Taxol). These strains were grown and then the amount of. a protein product in the Taxadiene pathway was measured. The data points are the mean values of protein product expression +/- standard deviation from four replicates 1. For the graph: a) Describe the graph: b) Describe the data: c) Interpret the data: Describe the statistics that are shown and what you can infer from them. Are there other statistics that would be better suited for this data? Is there any flaw in the way the data is represented?
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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For the graph:
a) Describe the graph:
b) Describe the data:
c) Interpret the data: Describe the statistics that are shown and what you can infer from them.
Transcribed Image Text:**Figure 3:**
**Graph Description:**
- The graph is a line chart that illustrates the relationship between the amount of recombinant DNA introduced and the mean level of expression (in arbitrary units) in two engineered *E. coli* strains (strain 17 and strain 22).
- The x-axis represents the "Amount of recombinant DNA introduced" with data points labeled as 1, 2, 3, 6, 11, and 21.
- The y-axis indicates the "Mean level of expression" measured in arbitrary units, ranging from 0 to 16.
- Two lines represent the two strains: strain 17 in red and strain 22 in blue.
**Graph Analysis:**
- For strain 17 (red line), the expression increases with the amount of recombinant DNA, reaching a mean level of 12 at the maximum DNA amount.
- For strain 22 (blue line), the expression also increases but at a slower rate, reaching a mean level of about 6.5 at the maximum DNA amount.
**Data Details:**
- The mean values are derived from protein product expression with a standard deviation from four replicates, indicating variability in the measurements.
**Figure 3 Explanation:**
Two engineered *E. coli* strains were developed with different recombinant DNA variations for genes involved in Taxadiene synthesis (a precursor for Taxol). The strains were grown, and the protein product in the Taxadiene pathway was quantified. Data points show mean protein product expression levels with a standard deviation from four replicates.
**Interpretation Task:**
1. Graph Description:
a) Describe the trend in expression levels.
b) Discuss the data's behavior and implications.
2. Data Interpretation:
a) Explain the statistics shown.
b) Assess if there are more suitable statistical methods for this data.
c) Evaluate any flaws in data representation.
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