Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:FIGURE
1.724 1.708
10 20 30 40 50 60 70 80
10 20 30 40 50 60 70 80
FRACTIONS
RADIOACTIVITY

Transcribed Image Text:Equilibrium Density Gradient Centrifugation of
DNA Samples
Review the following terms using the internet before working on the problem:
radioactive labeling, DNA, cesium chloride, equilibrium centrifugation, DNA denaturation
using heat
EΧPERIΜΕT
Radioactively labeled bacterial DNA was analyzed by cesium chloride (CSCI) equilibrium
density gradient centrifugation. The radioactivity of fractions collected from the gradients,
and the densities of the two peak fractions, were determined. Graph A: Native DNA sample;
Graph B: DNA sample heated to 100°C and rapidly cooled on ice before centrifugation.
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