A sample of purified DNA was incubated with deoxyribonuclease at 37°C. An aliquot was removed from the reaction mixture every minute for 5 minutes and the A260 recorded. The following data were obtained. Time (min) A260 0.60 1 0.64 0.67 0.70 0.72 0.73 4 Describe the action of deoxyribonuclease on DNA and explain the increase in A260.

A sample of purified DNA was incubated with deoxyribonuclease at 37°C. An aliquot was removed from the reaction mixture every minute for 5 minutes and the A260 recorded. The following data were obtained. Time (min) A260 0.60 1 0.64 0.67 0.70 0.72 0.73 4 Describe the action of deoxyribonuclease on DNA and explain the increase in A260.

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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